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Product Info Summary
| SKU: | M00780-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Primate |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Hu CD107a Purified Azide Free LAMP1 Monoclonal Antibody
SKU/Catalog Number
M00780-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD107a Purified Azide Free LAMP1 Monoclonal Antibody (Catalog# M00780-3). Tested in Flow Cytometry, WB, IHC-P, IHC-F, ICC application(s). This antibody reacts with Human, Primate, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD107a Purified Azide Free LAMP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
H4A3
Isotype
Mouse IgG1 kappa
Immunogen
Human PBMC. The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Reactive Species
M00780-3 is reactive to LAMP1 in Human, Mouse, Primate
Observed Molecular Weight
42 kDa
Calculated molecular weight
44.9 kDa
Background of LAMP1
CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00780-3 is guaranteed for Flow Cytometry, IHC-P, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1 μg/ml.
Immunohistochemistry: 2-8 μg/ml.
Flow cytometry: Intracellular and extracellular staining; recommended dilution: 1-12 µg/ml.
Validation Images & Assay Conditions
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Flow cytometry analysis (intracellular staining) of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.
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Immunohistochemistry staining of CD107a (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody H4A3 (anti-CD107a) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.
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Anti-Hu CD107a Purified (clone H4A3) works in WB application under reducing and non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of A431, THP-1, U87MG, and Jurkat cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody H4A3 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.
CD107a molecules were detected at 120 kDa in all tested cell lines under both reducing and non-reducing conditions.
Specific Publications For Anti-Hu CD107a Purified Azide Free LAMP1 Monoclonal Antibody (M00780-3)
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