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Product Info Summary
| SKU: | A01152-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL12B Antibody Picoband®
SKU/Catalog Number
A01152-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL12B Antibody Picoband® catalog # A01152-4. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL12B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01152-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL12B recombinant protein (Position: I23-E253).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01152-4 is reactive to Il12B in Human, Mouse
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.2 kDa
Background of Il12B
Subunit beta of interleukin 12 (IL12B), also known as interleukin 12B, or interleukin-12 subunit p40, is a subunit of human interleukin 12. It is mapped to 5q33.3. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01152-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse NIH3T3 cell, human A549 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of IL12B using anti-IL12B antibody (A01152-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse NIH3T3 cell lysates,
Lane 2: human A549 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.
Specific Publications For Anti-IL12B Antibody Picoband® (A01152-4)
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Customer Q&As
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6 Customer Q&As for Anti-IL12B Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-IL12B antibody A01152-4. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-22
Question
I see that the anti-IL12B antibody A01152-4 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-13
Question
Is there a BSA free version of anti-IL12B antibody A01152-4 available?
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL12B antibody A01152-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-31
Question
Is this A01152-4 anti-IL12B antibody reactive to the isotypes of IL12B?
R. Brown
Verified customer
Asked: 2016-05-27
Answer
The immunogen of A01152-4 anti-IL12B antibody is E. coli-derived human IL12B recombinant protein (Position: I23-E253). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-27
Question
I was wanting to use your anti-IL12B antibody for ELISA for human female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human female gonad identification?
S. Mitchell
Verified customer
Asked: 2016-01-26
Answer
It shows on the product datasheet, A01152-4 anti-IL12B antibody has been tested for ELISA, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human female gonad in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-26
Question
Will anti-IL12B antibody A01152-4 work for ELISA with female gonad?
A. Collins
Verified customer
Asked: 2015-02-18
Answer
According to the expression profile of female gonad, IL12B is highly expressed in female gonad. So, it is likely that anti-IL12B antibody A01152-4 will work for ELISA with female gonad.
Boster Scientific Support
Answered: 2015-02-18
