Anti-IL12 p40/IL12B Antibody
Rabbit IgG polyclonal antibody for Interleukin-12 subunit beta(IL12B) detection. Tested with WB in Human. Cited in 1 publication(s).
Anti-IL12 p40/IL12B Antibody Info At A Glance
|Product Name||Anti-IL12 p40/IL12B Antibody
See all Il12b primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for IL 12/IL12B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. IL 12/IL12B information: Molecular Weight: 37169 MW; Subcellular Localization: Secreted.|
|Cite This Product||Anti-IL12 p40/IL12B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1468)|
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|Anti-IL12 p40/IL12B Antibody may replace the following items: sc 1282|sc 1283|sc 365389|sc 374651|sc 4891|sc 53348|sc 53349|sc 57257|sc 65354|sc 74147|sc 74150|sc 7926.|
|Predicted Reactivity||Horse, Rabbit
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IL-12(p40)(23-40aa IWELKKDVYVVELDWYPD).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For IL12B (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3593|
|Species Of This Entry||Human|
|Protein Name||Interleukin-12 subunit beta|
|Alternative Names||Il12b|CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Acetylation; Glycosylation; Deacetylation; Methylation; Acylation; Ubiquitination|
|Gene Location||5q33.3, on Chromasome 5, gene sequence: NC_000005.10 (159314780..159330487, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL12B (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.|
|Interacting Proteins||IL12A; IL23A; Il23a|
|Related Diseases||Psoriasis; Crohn Disease; Autoimmune Reaction; Inflammation; Autoimmune Diseases; Diabetes Mellitus; Diabetes Mellitus, Insulin-dependent; Tuberculosis; Infective Disorder; Inflammatory Bowel Diseases; Arthritis; Mycobacterium Infections; Colitis; Cholangiocarcinoma; Ulcer; Dysequilibrium Syndrome; Intestinal Diseases; Malaria; Arthritis, Psoriatic; Ulcerative Colitis|
|Related Pathways||Pathogenesis; Immune Response; Secretion; Cytokine Production; Autophagy; Localization; Macrophage Activation; Inflammatory Response; Innate Immune Response; Cell Differentiation; Cell Activation; Complement Activation; Adaptive Immune Response; Virulence; Cellular Response To Dsrna; T Cell Activation; Interferon-gamma Production; Proteolysis; Hypersensitivity; Tropism|
|Background||IL-12, Interleukin 12, heterodimerizes with the IL12 p35 subunit to form IL12 and with the IL23 p19 subunit to form IL23. IL12b p 40 exists as a monomer and as a homodimer. This gene encodes a subunit of Interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The IL-12b gene contains 8 exons, the first and last of which are noncoding. By PCR analysis of DNA from rodent/human cell hybrids containing intact chromosomes or translocation chromosomes, the IL-12b gene is mapped to 5q31-q33.This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.|
|Scientific References||PMID: 1674604 by Gubler U., et al. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.|
PMID: 1673147 by Wolf S.F., et al. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells.
Our Boster Quality Guarantee for Anti-IL12 p40/IL12B Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human IL-12 Protein 10ng
Lane 2: Recombinant Human IL-12 Protein 5ng
Lane 3: Recombinant Human IL-12 Protein 2.5ng
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