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Product Info Summary
| SKU: | A00001T18 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-p53 (T18) TP53 Antibody
SKU/Catalog Number
A00001T18
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-p53 (T18) TP53 Antibody catalog # A00001T18. Tested in IF, WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p53 (T18) TP53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00001T18)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Thr18. AA range:1-50
Reactive Species
A00001T18 is reactive to TP53 in Human, Mouse, Rat
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00001T18 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF 1:50-200
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-p53 (T18) Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from Ovary cancer and K562 cells, using p53 (Phospho-Thr18) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, p53 (phospho Thr18) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Phospho-p53 (T18) TP53 Antibody (A00001T18)
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