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Product Info Summary
| SKU: | P00001-6 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00001-6
BM4095 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal Antibody catalog # P00001-6. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001-6)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BDO-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-p53 (S392)
Reactive Species
P00001-6 is reactive to TP53 in Human, Mouse, Rat
Observed Molecular Weight
53 kDa
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00001-6 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human 293T whole cell, human Hela whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell
IHC: human breast tissue
Validation Images & Assay Conditions
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Western blot analysis of p53 using anti-p53 antibody (P00001-6).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p53 antigen affinity purified monoclonal antibody (Catalog # P00001-6) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p53 at approximately 53 kDa. The expected band size for p53 is at 44 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal Antibody (P00001-6)
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4 Customer Q&As for Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal Antibody
Question
We have observed staining in human blood. Any tips? Is anti-Phospho-p53 (S392) Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
From what I have seen in literature blood does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2020-01-10
Question
We were happy with the WB result of your anti-Phospho-p53 (S392) Rabbit Monoclonal antibody. However we have observed positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
N. Zhao
Verified customer
Asked: 2019-03-29
Answer
According to literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-03-29
Question
We ordered your anti-Phospho-p53 (S392) Rabbit Monoclonal antibody for IHC on colon last year. I am using rat, and We intend to use the antibody for IP next. I am interested in examining colon as well as blood in our next experiment. Could give a recommendation on which antibody would work the best for IP?
A. Parker
Verified customer
Asked: 2017-10-06
Answer
I have checked the website and datasheets of our anti-Phospho-p53 (S392) Rabbit Monoclonal antibody and I see that P00001-6 has been validated on rat in both IHC and IP. Thus P00001-6 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-06
Question
We are currently using anti-Phospho-p53 (S392) Rabbit Monoclonal antibody P00001-6 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
J. Wu
Verified customer
Asked: 2013-11-15
Answer
The anti-Phospho-p53 (S392) Rabbit Monoclonal antibody (P00001-6) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-15
