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- Table of Contents
SMAD3 antibody (P00059-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Immunofluorescent analysis of A549 cells treated with TGFβ , using Phospho-Smad3 (S423 + S425) Antibody.
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Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
SMAD3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SMAD3 antibody was positively detected by IHC in:
human gastric adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SMAD3 antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Recommend
Tested
Recommend
Mouse
Recommend
Product Information
Product Name
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody
View all SMAD3 Antibodies
View all SMAD3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AHO-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Smad3 (S423 + S425)
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
48.1 kDa
Cite This Product
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00059-1)
Protocols
Immunohistochemistry (IHC) protocol for SMAD3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SMAD3 antibody
Publications
Application:
Species:
The Regulatory Mechanism of MicroRNA-144-3p on Damage to Endometrial Epithelial Cells Exposed to Copper Ions In Vitro
Published: 2025-09-05
Journal: Current Medical Science
Application: WB
Impact Factor: 1.50
Species: Human
PMID: 40911181
Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1
Published: 2024-03-16
Journal: BIOORGANIC CHEMISTRY
Impact Factor: 4.50
PMID: 38503026
TGF-β1 activates neutrophil signaling and gene expression but not migration
Published: 2023-09-08
Journal: PLoS One
Application: WB
Impact Factor: 3.70
Species: Human
PMID: 37682817
Defining the Role of Mitochondrial Fission in Corneal Myofibroblast Differentiation
Published: 2022-04-01
Journal: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Application: WB
Impact Factor: 4.93
Species: Cat
PMID: 35377925
Baicalin promotes chondrocyte viability and the synthesis of extracellular matrix through TGF-β/Smad3 pathway in chondrocytes
Published: 2021-09-15
Journal: American Journal of Translational Research
Application: WB,IF
Impact Factor: 4.06
Species: Mouse
PMID: 34650772
Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper
Published: 2021-08-24
Journal: MOLECULAR HUMAN REPRODUCTION
Application: WB
Impact Factor: 4.03
Species: Human
PMID: 34427668
Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition
Published: 2021-05-13
Journal: Theranostics
Application: WB
Impact Factor: 11.56
Species: Human
PMID: 34093874
Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT
Published: 2020-10-09
Journal: Vascular
Application: WB
Impact Factor: 1.32
Species: Human
PMID: 33035151
Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling
Published: 2020-08-10
Journal: Cell Death & Disease
Application: WB
Impact Factor: 6.30
Species: Human
PMID: 32826874
Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression
Published: 2019-07-16
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.45
Species: Human
PMID: 31323274
Activation of Slit2/Robo1 Signaling Promotes Tumor Metastasis in Colorectal Carcinoma through Activation of the TGF-β/Smads Pathway
Published: 2019-06-25
Journal: Cells
Application: IHC
Impact Factor: 5.66
Species: Mouse
PMID: 31242633
Review
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Product Promise
We promise Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00059-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026