Pig porcine TGF Beta 1 ELISA Kit PicoKine™

Pig porcine TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Pig TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml.

Product Info Summary

SKU: EK0513-PO
Size: 96 wells/kit, with removable strips.
Reactive Species: Pig
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and urine.

Product Name

Pig porcine TGF Beta 1 ELISA Kit PicoKine™

See all TGFB1 products

SKU/Catalog Number

EK0513-PO

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Pig porcine TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Pig TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Pig porcine TGF Beta 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0513-PO)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A279-S390

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Reactive Species

EK0513-PO is reactive to TGFB1 in Pig samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK0513-PO is guaranteed for ELISA in Pig by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0513-PO-CAP Anti-Pig TGFB1 Pre-coated 96-well strip microplate 1
EK0513-PO-ST Pig TGFB1 Standard 2 vials, 10 ng/tube
EK0513-PO-DA Pig TGFB1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0810.1920.2900.4580.8221.1781.6422.033

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Pig porcine TGF Beta 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2611236228118425
Standard deviation1.275.7121.001.998.9733.15
CV (%)4.9%5.1%5.8%7.1%7.6%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 126272425251.114.4%
Sample 21121061001011044.764.5%
Sample 336230931731632621.016.4%
*number of samples for each test n=16.

Gene/Protein Information For TGFB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01137

Gene ID

7040

Gene Name

TGFB1

Full Name

Transforming growth factor beta-1 proprotein

Weight

44.341kDa

Superfamily

TGF-beta family

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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5 Customer Q&As for Pig porcine TGF Beta 1 ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

L. Mehta

Verified customer

Asked: 2020-12-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-12-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-04-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-04-19

Question

Q: how to thaw whole blood sample for TGFB1 ELISA after freezing?

M. Bhatt

Verified customer

Asked: 2020-04-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGFB1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: is it okay to use edta plasma as samples in Pig TGFB1 Picokine® ELISA Kit (Catalog # EK0513-PO)?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TGFB1 causing degradation of its protein structure. TGFB1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TGFB1 ELISA, treating samples with different anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-06-11

Question

Q: What is the optimal O.D. value for TGFB1 ELISA kit? I used your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGFB1 even though the O.D. values are not very high?

T. Wilson

Verified customer

Asked: 2017-06-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2017-06-18

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