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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0928)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S33-E251
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0928 is reactive to F3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
EZ0928 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0928-CA||Rabbit anti- human F3 polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0928-DA||Biotinylated goat anti- human F3 polyclonal antibody (Detection Antibody)||500 µl, 5 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0928-ST||Lyophilized recombinant human F3 standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Tissue factor/F3 EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For F3 (Source: Uniprot.Org, NCBI)
tissue factor family
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on F3, check out the F3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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