Anti-PKAc Purified PRKACA Monoclonal Antibody

Product Name

Anti-PKAc Purified PRKACA Monoclonal Antibody

View all PRKACA Antibodies

SKU/Catalog Number

M00653

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PKAc Purified PRKACA Monoclonal Antibody (Catalog# M00653). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-PKAc Purified PRKACA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00653)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

6D2.1

Isotype

Mouse IgG1

Immunogen

Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha). The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00653 is reactive to PRKACA in Human

Applications

M00653 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

40.59kDa

Background of PRKACA

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; proteinkinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit –; PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1 μg/ml; positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).

Validation Images & Assay Conditions

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Figure 1. Dot Blot validation of PRKACA using Anti-PKAc Purified PRKACA Monoclonal Antibody (M00653).

Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. 11-477-C100).
The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1: GST-Akt1
Lane 2: GST-Akt2
Lane 3: GST-Akt3
Lane 4: GST-PKAc alpha
Lane 5: GST-PKAc beta
Lane 6: GST-PKAc gamma
Lane 7: GST-MEK 1
Lane 8: GST

Gene/Protein Information For PRKACA (Source: Uniprot.org, NCBI)

Alternative Names

PKA C alpha/beta; PRKACA/B PRKACA CAFD1, PKACA, PPNAD4 protein kinase cAMP-activated catalytic subunit alpha cAMP-dependent protein kinase catalytic subunit alpha|PKA C-alpha|protein kinase A catalytic subunit|protein kinase, cAMP-dependent, alpha catalytic subunit|protein kinase, cAMP-dependent, catalytic, alpha

For more info on PRKACA, check out the PRKACA Infographic

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In this infographic, you will see the following information for PRKACA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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