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Product Info Summary
| SKU: | A05366-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKA Beta cat PRKACB Antibody
SKU/Catalog Number
A05366-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKA Beta cat PRKACB Antibody catalog # A05366-1. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKA Beta cat PRKACB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05366-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
A synthetic peptide of human PKA C-beta (PRKACB)(NP_002722.1).
Reactive Species
A05366-1 is reactive to PRKACB in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
40.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05366-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using PKA C-beta antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using PKA C-beta antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded human appendix using PKA C-beta antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Click image to see more details
Western blot analysis of extracts of various cell lines, using PKA C-beta antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Specific Publications For Anti-PKA Beta cat PRKACB Antibody (A05366-1)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-PKA Beta cat PRKACB Antibody
Question
Does A05366-1 contain the amino acid D324?
Verified customer
Asked: 2021-01-27
Answer
Yes, the Anti-PKA Beta Cat PRKACB Antibody (A05366-1) contains the amino acid D324.
Boster Scientific Support
Answered: 2021-01-28
