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Product Info Summary
| SKU: | A00702-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Plasminogen/PLG Antibody Picoband®
SKU/Catalog Number
A00702-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Plasminogen/PLG Antibody Picoband® catalog # A00702-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plasminogen/PLG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00702-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Plasminogen/PLG recombinant protein (Position: V98-P356).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00702-1 is reactive to PLG in Human, Mouse, Rat
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
90.6 kDa
Background of PLG
Plasmin is an important enzyme present in blood that degrades many blood plasma proteins, including fibrin clots. It is mapped to 6q26. The degradation of fibrin is termed fibrinolysis. In humans, the plasmin protein is encoded by the PLG gene. The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00702-1 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat |
| ELISA|0.1-0.5μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of PLG using anti-PLG antibody (A00702-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse spleen tissue lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLG antigen affinity purified polyclonal antibody (Catalog # A00702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLG at approximately 90-100 kDa. The expected band size for PLG is at 90-100 kDa.
Specific Publications For Anti-Plasminogen/PLG Antibody Picoband® (A00702-1)
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Customer Q&As
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5 Customer Q&As for Anti-Plasminogen/PLG Antibody Picoband®
Question
We have tried in the past anti-Plasminogen/PLG antibody for ELISA on milk last year. I am using mouse, and We want to use the antibody for WB next. We need examining milk as well as kidney in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
I viewed the website and datasheets of our anti-Plasminogen/PLG antibody and it appears that A00702-1 has been validated on mouse in both ELISA and WB. Thus A00702-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-24
Question
We are currently using anti-Plasminogen/PLG antibody A00702-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
L. Zhao
Verified customer
Asked: 2018-09-19
Answer
The anti-Plasminogen/PLG antibody (A00702-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-19
Question
We were content with the WB result of your anti-Plasminogen/PLG antibody. However we have been able to see positive staining in milk secreted using this antibody. Is that expected? Could you tell me where is PLG supposed to be expressed?
J. Li
Verified customer
Asked: 2018-05-15
Answer
According to literature, milk does express PLG. Generally PLG expresses in secreted. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2018-05-15
Question
We have seen staining in rat right lobe of liver. Do you have any suggestions? Is anti-Plasminogen/PLG antibody supposed to stain right lobe of liver positively?
O. Rodriguez
Verified customer
Asked: 2016-07-08
Answer
According to literature right lobe of liver does express PLG. According to Uniprot.org, PLG is expressed in right lobe of liver, liver, kidney, milk, among other tissues. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401
Boster Scientific Support
Answered: 2016-07-08
Question
We need using your anti-Plasminogen/PLG antibody for positive regulation of blood vessel endothelial cell migration studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
R. Kulkarni
Verified customer
Asked: 2016-02-11
Answer
We appreciate your inquiry. This A00702-1 anti-Plasminogen/PLG antibody is validated on human placenta tissue, k562 whole cell lysates, hepg2 whole cell lysates, rat thymus tissue, spleen tissue, lung tissue, mouse thymus tissue. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-02-11
