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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Plasminogen/PLG Antibody Picoband™
Boster Bio Anti-Plasminogen/PLG Antibody Picoband™ catalog # A00702-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Plasminogen/PLG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00702-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human Plasminogen/PLG recombinant protein (Position: V98-P356).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00702-1 is reactive to PLG in Human, Mouse, Rat
A00702-1 is guaranteed for ELISA, WB Boster Guarantee
Background of PLG
Plasmin is an important enzyme present in blood that degrades many blood plasma proteins, including fibrin clots. It is mapped to 6q26. The degradation of fibrin is termed fibrinolysis. In humans, the plasmin protein is encoded by the PLG gene. The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Direct ELISA|0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLG using anti-PLG antibody (A00702-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse spleen tissue lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLG antigen affinity purified polyclonal antibody (Catalog # A00702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLG at approximately 90-100 kDa. The expected band size for PLG is at 90-100 kDa.
Gene/Protein Information For PLG (Source: Uniprot.org, NCBI)
peptidase S1 family
DKFZp779M0222; EC 3.4.21; EC 18.104.22.168; plasmin; Plasminogen; Plg PLG plasminogen plasminogen|plasmin*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on PLG, check out the PLG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
A00702-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Dysfunctional fibrinolysis and cerebral venous thrombosis
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