Anti-Factor VII/F7 Antibody

Coagulation Factor VII antibody

Boster Bio Anti-Factor VII/F7 Antibody catalog # PA2060. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2060
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Factor VII/F7 Antibody

View all Coagulation Factor VII Antibodies

SKU/Catalog Number

PA2060

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Factor VII/F7 Antibody catalog # PA2060. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor VII/F7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2060)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII (441-453aa SQYIEWLQKLMRS).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA2060 is reactive to F7 in Human

Applications

PA2060 is guaranteed for WB Boster Guarantee

Background of Coagulation Factor VII

F7 (Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34 (Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown (Zur and Nemerson, 1981). Di Bitondo et al. (2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For F7 (Source: Uniprot.org, NCBI)

Uniprot ID

P08709

Gene ID

2155

Gene Name

F7

Full Name

Coagulation factor VII

Weight

51.594kDa

Superfamily

peptidase S1 family

Alternative Names

coagulation factor VII (serum prothrombin conversion accelerator); Coagulation Factor VII; EC 3.4.21; EC 3.4.21.21; Eptacog alfa; F7; FVII coagulation protein; proconvertin; Serum prothrombin conversion accelerator; SPCA F7 SPCA coagulation factor VII coagulation factor VII|FVII coagulation protein|coagulation factor VII (serum prothrombin conversion accelerator)|eptacog alfa|proconvertin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on F7, check out the F7 Infographic

F7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for F7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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5 Customer Q&As for Anti-Factor VII/F7 Antibody

Question

I have a question about product PA2060, anti-Factor VII/F7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2060 anti-Factor VII/F7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-03

Question

Is a blocking peptide available for product anti-Factor VII/F7 antibody (PA2060)?

T. Carter

Verified customer

Asked: 2019-01-21

Answer

We do provide the blocking peptide for product anti-Factor VII/F7 antibody (PA2060). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-21

Question

Would PA2060 anti-Factor VII/F7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-17

Answer

It shows on the product datasheet, PA2060 anti-Factor VII/F7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-17

Question

Will anti-Factor VII/F7 antibody PA2060 work on monkey WB with right lobe of liver?

C. Williams

Verified customer

Asked: 2017-06-22

Answer

Our lab technicians have not tested anti-Factor VII/F7 antibody PA2060 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Factor VII/F7 antibody PA2060 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right lobe of liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-22

Question

We are currently using anti-Factor VII/F7 antibody PA2060 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

J. Edwards

Verified customer

Asked: 2016-08-30

Answer

The anti-Factor VII/F7 antibody (PA2060) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-30

Size

Conjugation

Total: $315

SKU:PA2060

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PA2060
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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