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Product Info Summary
| SKU: | PA2060 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Factor VII/F7 Antibody Picoband®
SKU/Catalog Number
PA2060
BA3085-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Factor VII/F7 Antibody catalog # PA2060. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Factor VII/F7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2060)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2060 is reactive to F7 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.6 kDa
Background of F7
F7 (Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34 (Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown (Zur and Nemerson, 1981). Di Bitondo et al. (2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2060 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell, JURKAT Whole Cell, RAJI Whole Cell
Validation Images & Assay Conditions
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Anti-Factor VII antibody, PA2060, Western blotting
All lanes: Anti Factor VII (PA2060) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
Specific Publications For Anti-Factor VII/F7 Antibody Picoband® (PA2060)
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Customer Q&As
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5 Customer Q&As for Anti-Factor VII/F7 Antibody Picoband®
Question
I have a question about product PA2060, anti-Factor VII/F7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2060 anti-Factor VII/F7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
Is a blocking peptide available for product anti-Factor VII/F7 antibody (PA2060)?
T. Carter
Verified customer
Asked: 2019-01-21
Answer
We do provide the blocking peptide for product anti-Factor VII/F7 antibody (PA2060). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-21
Question
Would PA2060 anti-Factor VII/F7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
It shows on the product datasheet, PA2060 anti-Factor VII/F7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-17
Question
Will anti-Factor VII/F7 antibody PA2060 work on monkey WB with right lobe of liver?
C. Williams
Verified customer
Asked: 2017-06-22
Answer
Our lab technicians have not tested anti-Factor VII/F7 antibody PA2060 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Factor VII/F7 antibody PA2060 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-22
Question
We are currently using anti-Factor VII/F7 antibody PA2060 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
J. Edwards
Verified customer
Asked: 2016-08-30
Answer
The anti-Factor VII/F7 antibody (PA2060) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-30
