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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.|
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Mouse SerpinE2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse SerpinE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SerpinE2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1885)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S20-P397
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1885 is reactive to Serpine2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.
EK1885 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin E2/PN1
Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
|EK1885-CAP||Anti-Mouse Serpine2 Pre-coated 96-well strip microplate||1|
|EK1885-ST||Mouse Serpine2 Standard||2 vials, 50 ng/tube|
|EK1885-DA||Mouse Serpine2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse SerpinE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin E2/PN1, Dilution ratio of 1:10000, concentration in serum is 30-70ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SerpinE2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Serpin E2/PN1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Serpine2 (Source: Uniprot.Org, NCBI)
GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2 Serpine2|B230326M24Rik, P, PAI-1, PI-7, PI7, PN, PN-1, S, Spi4, ne|serine (or cysteine) peptidase inhibitor, clade E, member 2|glia-derived nexin|GDN|peptidase inhibitor 7|protease nexin 1|protease nexin I|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2|serine (or cysteine) proteinase inhibitor, clade E, member 2|serine protease-inhibitor 4|serpin E2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Serpine2, check out the Serpine2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpine2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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