Anti-PP2A-alpha PPP2CA Monoclonal Antibody

Product Name

Anti-PP2A-alpha PPP2CA Monoclonal Antibody

View all PP2A alpha Antibodies

SKU/Catalog Number

M01893

Size

100ul

Form

Liquid

Description

Boster Bio Anti-PP2A-alpha PPP2CA Monoclonal Antibody catalog # M01893. Tested in IF, WB applications. This antibody reacts with Bovine, Canine, Chicken, Human, Mouse, Rabbit, Swine, Zebrafish.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PP2A-alpha PPP2CA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01893)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

2D10F4

Isotype

IgG

Immunogen

Purified recombinant human PP2A-Calpha (N-terminal) protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01893 is reactive to PPP2CA in Bovine, Canine, Chicken, Human, Mouse, Rabbit, Swine, Zebrafish

Applications

M01893 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

35.594kDa

Background of PP2A alpha

Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IF 1:100-1:500

Validation Images & Assay Conditions

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Figure 2. Immunofluorescent staining data of PPP2CA using Anti-PP2A-alpha PPP2CA Monoclonal Antibody (M01893).

Immunofluorescence (IF) analysis of HeLa cells using PP2A-Calpha Monoclonal Antibody.

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Figure 1. Western blotting validation for Anti-PP2A-alpha PPP2CA Monoclonal Antibody M01893

Western Blot (WB) analysis using PP2A-Calpha Monoclonal Antibody against MCF7 cell lysate.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For PPP2CA (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform PPP2CA NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C protein phosphatase 2 catalytic subunit alpha serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isozyme|replication protein C

For more info on PPP2CA, check out the PPP2CA Infographic

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In this infographic, you will see the following information for PPP2CA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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