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- Table of Contents
DLG4 antibody (A02128) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of U937 cells using anti-PSD95/DLG4 antibody (A02128).
Overlay histogram showing U937 cells stained with A02128 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95/DLG4 Antibody (A02128, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U937 cells stained with A02128 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95/DLG4 Antibody (A02128, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PSD95/DLG4 Antibody Picoband®
DLG4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
DLG4 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DLG4 antibody was positively detected by FC in:
U937 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Recommend
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Bovine
Predicted
Predicted
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-PSD95/DLG4 Antibody Picoband®
View all DLG4 Antibodies
View all DLG4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PSD95/DLG4 recombinant protein (Position: D18-L711).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
99 kDa
Calculated Molecular Weight
80.5 kDa
Background of DLG4
DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.
Cite This Product
Anti-PSD95/DLG4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02128)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Mouse
100%
High Homology
Cow
99%
High Homology
Monkey
94%
High Homology
Zebrafish
84%
Moderate Homology
Drosophila
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DLG4 antibody
Flow Cytometry (FC) protocol for DLG4 antibody
Publications
Application:
Species:
Safflower Yellow Alleviates Cognitive Impairment in Mice by Modulating Cholinergic System Function, Oxidative Stress, and CREB/BDNF/TrkB Signaling Pathway
Published: 2024-10-24
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 39461389
Overexpression cdc42 attenuates isoflurane-induced neurotoxicity in developmental brain of rats
Published: 2017-06-20
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.47
PMID: 28642137
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Product Promise
We promise Anti-PSD95/DLG4 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02128
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026