Anti-PPAR alpha/PPARA Antibody

Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

SKU PA1412
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ICC, WB

Overview

Product Name Anti-PPAR alpha/PPARA Antibody
See all Ppara primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1412
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Polyclonal antibody for PPAR alpha/PPARA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PPAR alpha/PPARA information: Molecular Weight: 52347 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.
Cite This Product Anti-PPAR alpha/PPARA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1412)
Specificity Anti-PPAR alpha/PPARA Antibody (PA1412) reacts with Human, Mouse, Rat Ppara, in native form and recombinant. Superfamily members of Ppara are not reactive to PA1412.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-PPAR alpha/PPARA Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-PPAR alpha/PPARA Antibody (PA1412).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

/antibody/pa1412-1-WB-anti-ppar-alpha-antibody.jpg

Anti-PPAR alpha antibody, PA1412, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Cardiac Muscle Tissue Lysate
Lane 4: MM231 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SMMC Cell Lysate
Lane 7: HT1080 Cell Lysate
Lane 8: SW620 Cell Lysate

/antibody/pa1412-2-IHC-anti-ppar-alpha-antibody.jpg

Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/antibody/pa1412-3-IHC-anti-ppar-alpha-antibody.jpg

Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Human Mammary Cancer Tissue

/antibody/pa1412-4-IHC-anti-ppar-alpha-antibody.jpg

Anti-PPAR alpha antibody, PA1412, IHC(P)
IHC(P): Mouse Intestine Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P23204
Gene Name Ppara
Protein Name Peroxisome proliferator-activated receptor alpha
Tissue Specificity Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.
Alternative Names Peroxisome proliferator-activated receptor alpha;PPAR-alpha;Nuclear receptor subfamily 1 group C member 1;Ppara;Nr1c1, Ppar;
Subcellular Localization Nucleus .
Molecular Weight 52347 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

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Publishing with Anti-PPAR alpha/PPARA Antibody (PA1412)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

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In stock
Order Product
PA1412
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure
Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice
Cheng L, Ge M, Lan Z, Ma Z, Chi W, Kuang W, Sun K, Zhao X, Liu Y, Feng Y, Huang Y, Luo M, Li L, Zhang B, Hu X, Xu L, Liu X, Huo Y, Deng H, Yang J, Xi Q, Zhang Y, Siegenthaler JA, Chen L. Arch Toxicol. 2018 Jan;92(1):469-485. doi: 10.1007/s00204-01...
Yang X, Fu Y, Hu F, Luo X, Hu J, Wang G. Exp Mol Med. 2018 Jan 19;50(1):e431. doi: 10.1038/emm.2017.243. PIK3R3 regulates PPARα expression to stimulate fatty acid β-oxidation and decrease hepatosteatosis
Shi P, Zhang Xx, Zhang Z, Zhang Y, Wu B, Cheng S, Li A. Chemosphere. 2012 Jul;88(4):407-12. Doi: 10.1016/J.Chemosphere.2012.02.055. Epub 2012 Mar 24. Chronic Exposure To Contaminated Drinking Water Stimulates Ppar Expression In Mice Livers.
Li Jp, Chen S, Peng H, Zhou Jl, Fang Hs. Bioelectromagnetics. 2014 Apr;35(3):170-80. Doi: 10.1002/Bem.21833. Epub 2014 Jan 14. Pulsed Electromagnetic Fields Protect The Balance Between Adipogenesis And Osteogenesis On Steroid-Induced Osteonecrosis...
Zhou X, Zhang J, Xu C, Wang W. J Pharmacol Sci. 2014;126(4):344-50. Doi: 10.1254/Jphs.14173Fp. Epub 2014 Dec 2. Curcumin Ameliorates Renal Fibrosis By Inhibiting Local Fibroblast Proliferation And Extracellular Matrix Deposition.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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