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- Table of Contents
PPARA antibody (A00600-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Flow Cytometry analysis of HepG2 cells using anti-PPAR alpha/PPARA antibody (A00600-2).
Overlay histogram showing HepG2 cells stained with A00600-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR alpha/PPARA Antibody (A00600-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with A00600-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR alpha/PPARA Antibody (A00600-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PPAR alpha/PPARA Antibody Picoband®
PPARA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
PPARA antibody was positively detected by WB in:
human HepG2 whole cell, human Hela whole cell, human MCF-7 whole cell, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PPARA antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Sheep
Predicted
Predicted
Rabbit
Predicted
Predicted
Product Information
Product Name
Anti-PPAR alpha/PPARA Antibody Picoband®
View all PPARA Antibodies
View all PPARA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PPAR alpha/PPARA recombinant protein (Position: M1-L426).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
52 kDa
Calculated Molecular Weight
52.2 kDa
Background of PPARA
Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.
Cite This Product
Anti-PPAR alpha/PPARA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00600-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Cow
95%
High Homology
Dog
94%
High Homology
Sheep
94%
High Homology
Mouse
92%
High Homology
Rat
92%
High Homology
Rabbit
92%
High Homology
Zebrafish
72%
Moderate Homology
Drosophila
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PPARA antibody
Flow Cytometry (FC) protocol for PPARA antibody
Publications
Application:
Species:
Targeting Egfr-Mediated Cell Proliferation and Lipid Metabolism Separation Effectively Accelerate Liver Regeneration
Published: 2026-04-22
Journal: CELL PROLIFERATION
Application: WB
Impact Factor: 5.60
Species: Human
PMID: 42019507
Mucin alleviates HFD-induced obesity and MASLD via an Akkermansia muciniphila-associated mucin-Neu5Ac-PPARα signaling axis
Published: 2026-03-25
Journal: PHARMACOLOGICAL RESEARCH
Application: WB
Impact Factor: 10.50
Species: Mouse,Human
PMID: 41895417
Sea Buckthorn Pericarp Flavonoids Improve Diet-Induced Hyperlipidemia via Coordinated Modulation of Hepatic Lipid Metabolism and Gut Microbiota
Published: 2026-03-17
Journal: Foods
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 41897771
SOX4 Regulates Thermogenesis in Brown Adipose Tissue via Independent Complexes with EBF2 and PPARγ
Published: 2025-09-22
Journal: Advanced Biology
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 40977422
Camel-Milk-Derived Extracellular Vesicles Mitigate High-Fat-Diet-Induced Obesity by Enhancing Thermogenesis and Modulating Lipid Metabolism in Mice
Published: 2025-08-14
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 6.20
Species: Mouse
PMID: 40810347
Targeting inflammation and necroptosis in diabetic kidney disease: A novel approach via PPARα modulation
Published: 2025-04-01
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 4.70
Species: Mouse
PMID: 40174339
Alkaline Mineral Complex Water Attenuates Transportation-Induced Hepatic Lipid Metabolism Dysregulation by AMPKα-SREBP-1c/PPARα Pathways
Published: 2024-10-23
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Rat
PMID: 39518926
Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5
Published: 2024-07-11
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 39055493
TIR8 Protects Against Nonalcoholic Steatohepatitis by Antagonizing Lipotoxicity-induced PPARα Downregulation and Reducing the Sensitivity of Hepatocytes to LPS
Published: 2024-06-06
Journal: Translational Research
Application: IHC,WB
Impact Factor: 6.40
Species: Mouse,Human
PMID: 38851532
Protective effects and mechanism of Sangyu granule on acetaminophen-induced liver injury in mice
Published: 2024-05-01
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38701935
Sulfur mustard analog 2-chloroethyl ethyl sulfide increases triglycerides by activating DGAT1-dependent biogenesis and inhibiting PGC1ɑ-dependent fat catabolism in immortalized human bronchial epithelial cells
Published: 2022-09-28
Journal: TOXICOLOGY MECHANISMS AND METHODS
Application: WB
Impact Factor: 4.02
Species: Human
PMID: 36106344
Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism
Published: 2022-01-01
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.66
Species: Mouse,Human
PMID: 35104757
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Product Promise
We promise Anti-PPAR alpha/PPARA Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00600-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026