Click image to see more details
Product Info Summary
| SKU: | PA1859 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PTCH2 Antibody Picoband®
SKU/Catalog Number
PA1859
BA3827-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTCH2 Antibody catalog # PA1859. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTCH2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1859)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PTCH2, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1859 is reactive to PTCH2 in Human, Mouse
Observed Molecular Weight
50 kDa
Calculated molecular weight
130.5 kDa
Background of PTCH2
PTCH2 (patched 2), also known as PTC2, encodes a 1.203-amino acid putative transmembrane protein that is highly homologous to the PTCH product. The Patched-hedgehog signaling pathway is involved in establishing segment polarity and the anterior-posterior orientation of a number of developing appendages. The pathway is conserved from fly to vertebrates and has been implicated in a number of human neoplasms and developmental syndromes. Mutations in the human Patched gene are the basis of the nevoid basal cell carcinoma syndrome. With age, homozygous mutant male mice developed skin lesions consisting of alopecia and epidermal hyperplasia, suggesting a role for Ptch2 in adult epidermal homeostasis via Shh signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1859 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: HELA Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-PTCH2 antibody, PA1859, Western blotting
WB: HELA Cell Lysate
Click image to see more details
TGF-β1/SMAD3 promotes the nuclear translocation and expression
of GLI2. (A) SMAD3 and p-SMAD3 protein levels were obtained by western blot upon
primary PSC treated with single or combined TGF-β1 and SIS3 treatment for 4 days
(n = 4). β-actin was used to normalize protein levels. (B) mRNA levels of Gli1, Smo,
Ptch1, and Ptch2 in PSCs following 24-hour treatment with 10 ng/mL TGF-β1 (n = 3).
(C) Representative immunofluorescence images showing expression levels of GLI1
(red), SMO (green), and PTCH2 (yellow) in PSCs treated with 10 ng/mL TGF-β1 for
72 hours. Nuclei were stained with DAPI (blue). Scale bar: 20 μm. Fluorescence
intensity of GLI1, PTCH2, and SMO was quantified (n = 4). All data are represented
as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001,∗∗∗∗p < 0.0001. ns, not significant.
Index in PubMed under a CC BY license. PMID: 41281756
Specific Publications For Anti-PTCH2 Antibody Picoband® (PA1859)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PTCH2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PTCH2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
2 Customer Q&As for Anti-PTCH2 Antibody Picoband®
Question
We are currently using anti-PTCH2 antibody PA1859 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-PTCH2 antibody (PA1859) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
Is PA1859 suitable for staining cells and/or tissue sections?
Verified customer
Asked: 2019-05-07
Answer
Our lab technicians have tested the Anti-PTCH2 Antibody (PA1859) for staining cells and/or tissue sections and it did not test well for these applications. So, we only recommend using this antibody for Western Blot application.
Boster Scientific Support
Answered: 2019-05-07
