Anti-Guanylate-binding protein 1 GBP1 Antibody
Rabbit IgG polyclonal antibody for Guanylate-binding protein 1(GBP1) detection. Tested with WB in Human.
Anti-Guanylate-binding protein 1 GBP1 Antibody Info At A Glance
|Product Name||Anti-Guanylate-binding protein 1 GBP1 Antibody
See all GBP1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GBP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. GBP1 information: Molecular Weight: 67931 MW; Subcellular Localization: Cytoplasm. Golgi apparatus membrane; Lipid- anchor; Cytoplasmic side. Secreted. Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of interferon-gamma induction. Golgi membrane localization requires isoprenylation and the presence of another IFN-gamma-induced factor.|
|Cite This Product||Anti-Guanylate-binding protein 1 GBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1550)|
|Similar Products From
|Anti-Guanylate-binding protein 1 GBP1 Antibody may replace the following items: sc 10579|sc 10586|sc 53857.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GBP1(566-589aa QKESRIMKNEIQDLQTKMRRRKAC).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GBP1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2633|
|Species Of This Entry||Human|
|Protein Name||Guanylate-binding protein 1|
|Superfamily||TRAFAC class dynamin-like GTPase superfamily|
|Alternative Names||GBP1|GBP-1; GTP-binding protein 1; Guanine nucleotide-binding protein 1; guanylate binding protein 1, interferon-inducible; guanylate binding protein 1, interferon-inducible, 67kDa; huGBP-1; interferon-induced guanylate-binding protein 1|
|Post Tranlational Modifications||Phosphorylation; Prenylation; Farnesylation; Biotinylation; Sumoylation; Cleavage; Acetylation|
|Subcellular Localization||Cytoplasm. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cell membrane. Secreted. Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.|
|Gene Location||1p22.2, on Chromasome 1, gene sequence: NC_000001.11 (89052319..89065208, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GBP1 (Source: Uniprot.org, NCBI)
|Protein Function||Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.|
|Related Diseases||Inflammation; Neoplasms; Tumor Angiogenesis; Malignant Neoplasms; Glioma; Pathologic Neovascularization; Adenocarcinoma; Carcinoma; Virus Diseases; Cell Invasion; Glioblastoma; Vesicular Stomatitis; Stomatitis; Hypoxia; Sarcoma; Influenza; Hepatitis; Prostatic Neoplasms; Malignant Neoplasm Of Ovary; Colorectal Cancer|
|Related Pathways||Angiogenesis; Cell Proliferation; Localization; Immune Response; Endothelial Cell Proliferation; Cell Death; Transport; Response To Interferon-gamma; Cell Growth; Pathogenesis; Cell Migration; Virulence; Myelination; Chemotaxis; Glucose Transport; Prenylation; Macrophage Activation; Drug Resistance; Cellular Localization; Secretion|
|Background||Interferons induce a large number of genes in their target cells, including those coding for guanylate-binding proteins(GBPs). GBPs, such as GBP1, are characterized by their ability to specifically bind guanine nucleotides(GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. The 593-amino acid GBP1 protein shares 77% and 88% identity with GBP2 and GBP3, respectively. All GBPs, including GBP1, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases. The GBP1 gene to the GBP gene cluster on chromosome 1p22.2. It is located telomeric to GBP2 and centromeric to GBP3. High expression of GBP1, GBP2, and GBP3 are in endothelial cells after stimulation with IFNG, TNF, or IL1B .|
|Scientific References||PMID: 1715024 by Cheng Y.-S.E., et al. Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP.|
PMID: 8830800 by Nantais D.E., et al. Prenylation of an interferon-gamma-induced GTP-binding protein: the human guanylate binding protein, huGBP1.
Our Boster Quality Guarantee for Anti-Guanylate-binding protein 1 GBP1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti GBP1 (PA1550) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
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