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Product Info Summary
| SKU: | M03708 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RbAp46 (RBBP7) Mouse Monoclonal Antibody [Clone ID: OTI5A4]
SKU/Catalog Number
M03708
Size
100 µl
Description
Boster Bio RBBP7 (RbAp46) mouse monoclonal antibody, clone OTI5A4 (formerly 5A4). Catalog# M03708. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-RbAp46 (RBBP7) Mouse Monoclonal Antibody [Clone ID: OTI5A4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03708)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5A4
Isotype
IgG1
Immunogen
Full length human recombinnat protein of RBBP7 (NP_002884) produced in HEK293T cell.
Reactive Species
M03708 is reactive to RBBP7 in Human, Mouse, Rat
Calculated molecular weight
47.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03708 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBBP7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP7.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and RBBP7-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-RBBP7 monoclonal antibody M03708. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500).
Specific Publications For Anti-RbAp46 (RBBP7) Mouse Monoclonal Antibody [Clone ID: OTI5A4] (M03708)
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