Introduction
B lymphoid kinase (BLK) is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains which contain consensus sequences of the Src protein tyrosine kinase family. BLK is a nonreceptor tyrosine-kinase belonging to the src family of proto-oncogenes which are typically involved in cell proliferation and differentiation. The BLK protein is expressed specifically in the B cell lineage and has a role in the signal-transduction pathway which is restricted to B lymphoid cells. In addition, BLK stimulates insulin synthesis and secretion in response to glucose and increases the expression of several pancreatic beta-cell transcription factors.
Overview
| Product Name |
Human CYR61/CCN1 ELISA Kit PicoKine™
See all Cyr61/CCN1 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK1203 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human CYR61 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CYR61 tissue specificity: |
| Cite This Product |
Human CYR61/CCN1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1203)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk. |
| Sensitivity |
<10pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: CHO; Immunogen sequence: A22-D381 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For CYR61 (Source: Uniprot.org, NCBI)
| Uniprot Id | O00622 |
|---|
| NCBI Gene Id | 3491 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | CYR61 |
|---|
| Protein Name | CCN family member 1 |
|---|
| Superfamily | CCN family |
|---|
| Alternative Names | Cyr61/CCN1|CCN family member 1; CCN1; CCN1IGF-binding protein 10; Cyr61; Cysteine-rich angiogenic inducer 61; cysteine-rich, angiogenic inducer, 61; GIG1; GIG1cysteine-rich heparin-binding protein 61; IBP-10; IGFBP-10; IGFBP10cysteine-rich, anigogenic inducer, 61; Insulin-like growth factor-binding protein 10; protein CYR61; Protein GIG1 |
|---|
| Molecular Weight | 42027 |
|---|
| Post Tranlational Modifications | Phosphorylation; Reduction; Cleavage; Acetylation; Dephosphorylation; Demethylation; Biotinylation; Deacetylation; Glycosylation; Methylation; Geranylgeranylation |
|---|
| Subcellular Localization | Secreted. |
|---|
| Gene Location | 1p22.3, on Chromasome 1, gene sequence: NC_000001.11 (85580761..85583950) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CYR61 (Source: Uniprot.org, NCBI)
| Protein Function | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
|---|
| Related Diseases | Malignant Neoplasms; Neoplasms; Tissue Adhesions; Tumor Angiogenesis; Carcinoma; Pathologic Neovascularization; Neoplasm Metastasis; Cell Transformation, Neoplastic; Malignant Neoplasm Of Breast; Cell Invasion; Inflammation; Nephroblastoma; Mammary Neoplasms; Neoplasm Invasiveness; Fibrosis; Hypoxia; Hyperplasia; Adenocarcinoma; Malignant Paraganglionic Neoplasm |
|---|
| Related Pathways | Angiogenesis; Cell Proliferation; Cell Adhesion; Cell Migration; Cell Growth; Pathogenesis; Wound Healing; Localization; Secretion; Cell Cycle; Chemotaxis; Cell Death; Cell Differentiation; Reverse Transcription; Rna Interference; Cell Motility; Aging; Translation; Regeneration; Tube Formation |
|---|
| Background | Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers. |
|---|
| Scientific References | PMID: 9135077 by Jay P., et al. The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p.
PMID: 9536281 by Martinerie C., et al. Chromosomal mapping and expression of the human cyr61 gene in tumour cells from the nervous system. |
|---|
Kit Components And Assay QC Data Details Of Human CYR61/CCN1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK1203-CAP | 96-well plate precoated with anti-Human Cyr61/CCN1 antibody | 1 |
| EK1203-ST | lyophilized recombinant Human Cyr61/CCN1 standard | 10ng/tube |
| EK1203-DA | biotinylated anti-Human Cyr61/CCN1 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1203 Human CYR61/CCN1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.137 | 0.214 | 0.375 | 0.722 | 1.097 | 1.734 | 2.209 |
We measured random samples of Human CYR61/CCN1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 61 | 248 | 814 | 59 | 242 | 806 |
| Standard deviation | 2.86 | 16.12 | 55.35 | 3.3 | 17.66 | 64.48 |
| CV(%) | 4.7% | 6.5% | 6.8% | 5.6% | 7.3% | 8% |
We measured the performance consistency of Human CYR61/CCN1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 61 | 64 | 63 | 58 | 61 | 2.29 | 3.7% |
| Sample 2 | 248 | 273 | 263 | 255 | 259 | 9.31 | 3.5% |
| Sample 3 | 814 | 907 | 783 | 869 | 843 | 47.99 | 5.6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Cyr61/CCN1 primary antibodies,
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