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Product Info Summary
| SKU: | PA2177 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Egr1 Antibody Picoband®
SKU/Catalog Number
PA2177
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Egr1 Antibody catalog # PA2177. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Egr1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2177)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Egr1, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2177 is reactive to EGR1 in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
57.5 kDa
Background of EGR1
Earlu growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2177 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-Egr1 Antibody Picoband® (PA2177)
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Customer Q&As
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5 Customer Q&As for Anti-Egr1 Antibody Picoband®
Question
I was wanting to use your anti-Egr1 antibody for WB for human pns on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pns identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
As indicated on the product datasheet, PA2177 anti-Egr1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pns in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this PA2177 anti-Egr1 antibody reactive to the isotypes of EGR1?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The immunogen of PA2177 anti-Egr1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human EGR1(23-43aa FPHSPTMDNYPKLEEMMLLSN), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-Egr1 antibody PA2177 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The anti-Egr1 antibody (PA2177) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
Does PA2177 anti-Egr1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
You can see on the product datasheet, PA2177 anti-Egr1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-21
Question
I see that the anti-Egr1 antibody PA2177 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-11-28
