Mouse WISP1/CCN4 ELISA Kit PicoKine®

WISP-1/CCN4 ELISA kit for Mouse

Mouse WISP1/CCN4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccn4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1195
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse WISP1/CCN4 ELISA Kit PicoKine®

View all WISP-1/CCN4 ELISA kits

SKU/Catalog Number

EK1195

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse WISP1/CCN4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccn4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse WISP1/CCN4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1195)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T23-N367

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1195 is reactive to Ccn4 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1195 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of WISP-1/CCN4

WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

Kit Components

Catalog Number Description Quantity
EK1195-CAP Anti-Mouse Ccn4 Pre-coated 96-well strip microplate 1
EK1195-ST Mouse Ccn4 Standard 2 vials, 10 ng/tube
EK1195-DA Mouse Ccn4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0670.2560.3200.4990.9251.3851.9472.393

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect WISP-1/CCN4, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse WISP1/CCN4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6626187869265906
Standard deviation4.2916.760.585.5818.8167.95
CV (%)6.5%6.4%6.9%8.1%7.1%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of WISP-1/CCN4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 166727273702.773.9%
Sample 226122422025524018.177.5%
Sample 387887788190588511.491.2%
*number of samples for each test n=16.

Gene/Protein Information For Ccn4 (Source: Uniprot.Org, NCBI)

Uniprot ID

O54775

Gene ID

22402

Gene Name

Ccn4

Full Name

CCN family member 4

Weight

40.703kDa

Superfamily

CCN family

Alternative Names

CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1 Ccn4|AW146261, CCN, Elm, Elm1, Wi, Wisp1|cellular communication network factor 4|CCN family member 4|WNT1 inducible signaling pathway protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccn4, check out the Ccn4 Infographic

Ccn4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccn4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Mouse WISP1/CCN4 ELISA Kit PicoKine®

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Wisp1 using the EK1195 Mouse Wisp1 Picokine® ELISA Kit?

W. Robinson

Verified customer

Asked: 2020-07-06

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2020-07-06

Question

Q: how to thaw whole blood sample for Wisp1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Wisp1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-03-21

Question

Q: if the enzyme conjugated Wisp1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Wisp1 antigen?

D. Roberts

Verified customer

Asked: 2018-02-18

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-02-18

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