Anti-RPA Interacting Protein Antibody

Product Name

Anti-RPA Interacting Protein Antibody

View all RPAIN Antibodies

SKU/Catalog Number

A11439

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RPA Interacting Protein Antibody (Catalog # A11439). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RPA Interacting Protein antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RPA Interacting Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11439)

Host

Rabbit

Contents

RPA Interacting Protein Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RPA IP antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RPA IP. The immunogen is located within the last 50 amino acids of RPA Interacting Protein.

Reactive Species

A11439 is reactive to RPAIN in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

24.8 kDa

Background of RPAIN

Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11439 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RPAIN

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