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- Table of Contents
S100B antibody (M00979-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-S100B/S100 Beta Rabbit Monoclonal Antibody
S100B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
S100B antibody was positively detected by WB in:
rat brain tissue, rat C6 whole cell, mouse brain tissue, A375 cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
S100B antibody was positively detected by IHC in:
Mouse pancreas tissue, Rat cerebral cortex tissue, Mouse hippocampus tissue, Human testis cancer tissue, Human glioblastoma tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
S100B antibody was positively detected by IF in:
rat C6 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Rat
Tested
Tested
Recommend
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Human
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-S100B/S100 Beta Rabbit Monoclonal Antibody
View all S100B Antibodies
View all S100B Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S100B
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
11 kDa
Calculated Molecular Weight
10.7 kDa
Cite This Product
Anti-S100B/S100 Beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00979-1)
Protocols
Western Blot protocol for S100B antibody
Immunohistochemistry (IHC) protocol for S100B antibody
Immunofluorescence (IF) protocol for S100B antibody
Publications
Application:
Species:
Gene cloning of S100β and NGF and localization of their expression in the small intestine of broilers of various ages
Published: 2025-09-26
Journal: Scientific Reports
Application: IHC,WB
Impact Factor: 3.90
Species: Broiler
PMID: 41006582
Effect of TGF- β 2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia
Published: 2022-09-12
Journal: Biomed Research International
Application: ICC
Impact Factor: 3.25
Species: Guinea pig
PMID: 36132076
SerpinA3N attenuates ischemic stroke injury by reducing apoptosis and neuroinflammation
Published: 2021-12-12
Journal: CNS Neuroscience & Therapeutics
Application: IF
Impact Factor: 5.24
Species: Mouse
PMID: 34897996
Folic acid contributes to peripheral nerve injury repair by promoting Schwann cell proliferation, migration, and secretion of nerve growth factor
Published: 2019-01-01
Journal: Neural Regeneration Research
Application: IF,IHC
Impact Factor: 2.47
Species: Rat
PMID: 30531087
Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress
Published: 2018-04-04
Journal: Gastroenterology Research and Practice
Application: IF,IHC
Impact Factor: 1.86
Species: Human,Rat
PMID: 29849603
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord
Published: 2018-01-11
Journal: BIOMATERIALS
Application: IF
Impact Factor: 8.81
Species: Rat
PMID: 29353106
Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Published: 2017-01-05
Journal: Scientific Reports
Application: WB,IF,IHC
Impact Factor: 4.26
Species: Rat
PMID: 28054637
Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury
Published: 2015-02-15
Journal: Purinergic Signalling
Application: IF
Impact Factor: 3.89
Species: Rat
PMID: 25682129
Cotransplantation of Olfactory Ensheathing Cells and Schwann Cells Combined with Treadmill Training Promotes Functional Recovery in Rats with Contused Spinal Cords
Published: 2013-01-01
Journal: CELL TRANSPLANTATION
Application: IHC
Impact Factor: 4.42
Species: Rat
PMID: 24044361
Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia
Published: 2012-06-06
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: ICC
Impact Factor: 1.57
Species: Rat
PMID: 22684116
Biological characteristics of a specific brain metastatic cell line derived from human lung adenocarcinoma
Published: 2009-08-08
Journal: MEDICAL ONCOLOGY
Application: WB,IHC
Impact Factor: 1.13
Species: Human
PMID: 19669609
17β-estradiol regulates the numbers, endocytosis, stimulative capacity and IL-10 secretion of mouse spleen dendritic cells
Published: 2004-11-24
Journal: TOXICOLOGY LETTERS
Impact Factor: 2.22
PMID: 15603918
Review
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Product Promise
We promise Anti-S100B/S100 Beta Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00979-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026