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Product Info Summary
| SKU: | M00979-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-S100B/S100 Beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M00979-1
BM4087 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S100B/S100 Beta Rabbit Monoclonal Antibody catalog # M00979-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100B/S100 Beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00979-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BCB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S100B
Reactive Species
M00979-1 is reactive to S100B in Human, Mouse, Rat
Observed Molecular Weight
11 kDa
Calculated molecular weight
10.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00979-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: rat brain tissue, rat C6 whole cell, mouse brain tissue, A375 cell
IHC: Mouse pancreas tissue, Rat cerebral cortex tissue, Mouse hippocampus tissue, Human testis cancer tissue, Human glioblastoma tissue, rat brain tissue
IF: rat C6 cell
Validation Images & Assay Conditions
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Western blot analysis of S100B using anti-S100B antibody (M00979-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse brain tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100B antigen affinity purified monoclonal antibody (Catalog # M00979-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100B at approximately 11 kDa. The expected band size for S100B is at 11 kDa.
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Western blot analysis of S100B expression in A375 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Mouse pancreas, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse hippocampus , using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:250 dilution.
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Immunohistochemical analysis of paraffin-embedded rat brain, using S100B Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of A375 cells, using S100B Antibody .
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IF analysis of S100B using anti-S100B antibody (M00979-1) .
S100B was detected in an immunocytochemical section of rat C6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-S100B Antibody (M00979-1) at a dilution of 10 ug/mL overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-S100B/S100 Beta Rabbit Monoclonal Antibody (M00979-1)
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-S100B/S100 Beta Rabbit Monoclonal Antibody
Question
I am looking for using your anti-S100B/S100 Beta Rabbit Monoclonal antibody for central nervous system development studies. Has this antibody been tested with western blotting on a375 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
I appreciate your inquiry. This M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody is tested on a375 cells. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-05-06
Question
Does M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
As indicated on the product datasheet, M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-10
Question
Here is the WB image, lot number and protocol we used for skin using anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
My question regarding product M00979-1, anti-S100B/S100 Beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Gonzalez
Verified customer
Asked: 2020-01-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-29
Question
Do you have a BSA free version of anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 available?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-S100B/S100 Beta Rabbit Monoclonal antibody for WB for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
It shows on the product datasheet, M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
Is this M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody reactive to the isotypes of S100B?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The immunogen of M00979-1 anti-S100B/S100 Beta Rabbit Monoclonal antibody is A synthesized peptide derived from human S100B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-11
Question
We were happy with the WB result of your anti-S100B/S100 Beta Rabbit Monoclonal antibody. However we have seen positive staining in c1 segment of cervical spinal cord cytoplasm. using this antibody. Is that expected? Could you tell me where is S100B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
From what I have seen in literature, c1 segment of cervical spinal cord does express S100B. Generally S100B expresses in cytoplasm. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-24
Question
I see that the anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 works with WB, what is the protocol used to produce the result images on the product page?
J. Huang
Verified customer
Asked: 2019-09-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-16
Question
Have M00979-1 and M00213-1 been tested for Flow Cytometry application?
Verified customer
Asked: 2019-07-04
Answer
The Anti-S100B/S100 Beta Rabbit Monoclonal Antibody (M00979-1) and Anti-GFAP Rabbit Monoclonal Antibody (M00213-1) can't work for Flow Cytometry application.
Boster Scientific Support
Answered: 2019-07-05
Question
Can FITC or Alexfluor 488 secondary antibodies work for M00979-1 and M00213-1?
Verified customer
Asked: 2019-07-04
Answer
FITC or Alexfluor 488 secondary antibodies could work for the Anti-S100B/S100 Beta Rabbit Monoclonal Antibody (M00979-1) and Anti-GFAP Rabbit Monoclonal Antibody (M00213-1).
Boster Scientific Support
Answered: 2019-07-05
Question
We want to test anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 on human skin for research purposes, then I may be interested in using anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
The products we sell, including anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-21
Question
We have seen staining in mouse c1 segment of cervical spinal cord. Any tips? Is anti-S100B/S100 Beta Rabbit Monoclonal antibody supposed to stain c1 segment of cervical spinal cord positively?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Based on literature c1 segment of cervical spinal cord does express S100B. Based on Uniprot.org, S100B is expressed in c1 segment of cervical spinal cord, skin, among other tissues. Regarding which tissues have S100B expression, here are a few articles citing expression in various tissues:
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-10-03
Question
Would anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 work for WB with skin?
S. Krishna
Verified customer
Asked: 2018-07-05
Answer
According to the expression profile of skin, S100B is highly expressed in skin. So, it is likely that anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 will work for WB with skin.
Boster Scientific Support
Answered: 2018-07-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1. Let me know if you need anything else.
B. Li
Verified customer
Asked: 2018-02-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-06
Question
We are currently using anti-S100B/S100 Beta Rabbit Monoclonal antibody M00979-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
The anti-S100B/S100 Beta Rabbit Monoclonal antibody (M00979-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-13
Question
Is a blocking peptide available for product anti-S100B/S100 Beta Rabbit Monoclonal antibody (M00979-1)?
D. Evans
Verified customer
Asked: 2017-03-09
Answer
We do provide the blocking peptide for product anti-S100B/S100 Beta Rabbit Monoclonal antibody (M00979-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-03-09
Question
We ordered your anti-S100B/S100 Beta Rabbit Monoclonal antibody for IHC on c1 segment of cervical spinal cord in a previous experiment. I am using human, and We intend to use the antibody for IF next. We want examining c1 segment of cervical spinal cord as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
K. Jones
Verified customer
Asked: 2015-08-06
Answer
I looked at the website and datasheets of our anti-S100B/S100 Beta Rabbit Monoclonal antibody and it appears that M00979-1 has been validated on human in both IHC and IF. Thus M00979-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-08-06
