Anti-SCARB1 Antibody

Product Name

Anti-SCARB1 Antibody

View all SCARB1 Antibodies

SKU/Catalog Number

A01093

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SCARB1 Antibody (Catalog # A01093). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SCARB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SCARB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01093)

Host

Rabbit

Contents

SCARB1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SCARB1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SCARB1. The immunogen is located within amino acids 70 - 120 of SCARB1.

Cross-reactivity

At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms.

Reactive Species

A01093 is reactive to SCARB1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

60.9 kDa

Background of SCARB1

Scavenger receptor class B member 1 (SCARB1), also known as SR-BI, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARB1 is a multi-ligand cell-surface receptor that mediates the selective uptake of lipid from HDL cholesterol into cells and is expressed in steroidogenic tissues in adult animals. Other ligands of SCARB1 include native, acetylated, or oxidized LDL and anionic phospholipids. SCARB1-deficient mice have elevated HDL levels and increased susceptibility to atherosclerosis on fat feeding, indicating its importance in the regulation of cholesterol homeostasis. Along with CLDN1, LDL-R, and the tetraspanin superfamily member CD81, SCARB1 has been reported to be an entry factor for the Hepatitis C virus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01093 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SCARB1 antibody can be used for detection of SCARB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SCARB1

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