Product Info Summary
SKU: | A00497 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SF2 SRSF1 Antibody
SKU/Catalog Number
A00497
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SF2 SRSF1 Antibody catalog # A00497. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SF2 SRSF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00497)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SC6A4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00497 is reactive to SRSF1 in Human, Mouse, Rat
Applications
A00497 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
27.745kDa
Background of SF2
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of SRSF1 using Anti-SF2 SRSF1 Antibody (A00497).
ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-SF2 SRSF1 Antibody A00497
Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution.Positive control:
Lane 1: Mouse heart
Lane 2: Mouse liver
Lane 3: K562
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SRSF1 (Source: Uniprot.org, NCBI)
Gene Name
SRSF1
Full Name
Serine/arginine-rich splicing factor 1
Weight
27.745kDa
Superfamily
splicing factor SR family
Alternative Names
Alternative-splicing factor 1; ASFpre-mRNA-splicing factor SF2, P33 subunit; MGC5228; serine/arginine-rich splicing factor 1; SF2FLJ53078; SF2p33; SFRS1splicing factor 2; Splicing factor, arginine/serine-rich 1ASF-1; SR splicing factor 1; SRp30a SRSF1 ASF, SF2, SF2p33, SFRS1, SRp30a serine and arginine rich splicing factor 1 serine/arginine-rich splicing factor 1|SR splicing factor 1|alternative-splicing factor 1|pre-mRNA-splicing factor SF2, P33 subunit|splicing factor 2|splicing factor, arginine/serine-rich, 30-KD, A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRSF1, check out the SRSF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRSF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SF2 SRSF1 Antibody (A00497)
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No publications found for A00497
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