Anti-SHANK3 Antibody

Product Name

Anti-SHANK3 Antibody

View all SHANK3 Antibodies

SKU/Catalog Number

A01231

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SHANK3 Antibody (Catalog # A01231). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SHANK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01231)

Host

Rabbit

Contents

SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3. The immunogen is located within amino acids 890 - 940 of SHANK3.

Cross-reactivity

At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.

Reactive Species

A01231 is reactive to SHANK3 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

184.7 kDa

Background of SHANK3

SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01231 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SHANK3

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