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SLC22A3 antibody (A04914-1) performs well in Flow Cytometry, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (A04914-1).
Overlay histogram showing Hela cells stained with A04914-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (A04914-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A04914-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (A04914-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SLC22A3 Antibody Picoband®
SLC22A3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, WB |
Tested Applications
SLC22A3 antibody was positively detected by WB in:
human HEK293 whole cell, human Hela whole cell, mouse skeletal muscle tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC22A3 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Mouse
Tested
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Product Information
Product Name
Anti-SLC22A3 Antibody Picoband®
View all SLC22A3 Antibodies
View all SLC22A3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC22A3, which shares 76.5% amino acid (aa) sequence identity with both mouse and rat SLC22A3.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
61 kDa
Calculated Molecular Weight
61.3 kDa
Background of SLC22A3
Solute carrier family 22 (extraneuronal monoamine transporter), member 3, also known as SLC22A3 or OCT3 is a protein that in humans is encoded by the SLC22A3 gene. This gene is mapped to 6q25.3. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene mediates potential-dependent transport of a variety of organic cations. It may play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
Cite This Product
Anti-SLC22A3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04914-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Monkey
94%
High Homology
Mouse
76%
Moderate Homology
Rat
76%
Moderate Homology
Zebrafish
53%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC22A3 antibody
Flow Cytometry (FC) protocol for SLC22A3 antibody
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Product Promise
We promise Anti-SLC22A3 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A04914-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026