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- Table of Contents
SLIRP antibody (A08297-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A549 cells using anti-SLIRP antibody (A08297-1).
Overlay histogram showing A549 cells stained with A08297-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLIRP Antibody (A08297-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with A08297-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLIRP Antibody (A08297-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SLIRP Antibody Picoband®
SLIRP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
SLIRP antibody was positively detected by WB in:
human Caco-2 whole cell, human MCF-7 whole cell, human PC-3 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLIRP antibody was positively detected by IHC in:
human colon cancer tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLIRP antibody was positively detected by IF in:
human colon cancer tissue, human stomach cancer tissue
SLIRP antibody was positively detected by ICC/IF in:
A549 cell
SLIRP antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-SLIRP Antibody Picoband®
View all SLIRP Antibodies
View all SLIRP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SLIRP recombinant protein (Position: A11-F109). Human SLIRP shares 77.8% amino acid (aa) sequence identity with mouse SLIRP.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
15 kDa
Calculated Molecular Weight
12.3 kDa
Background of SLIRP
Steroid receptor RNA activator (SRA, or SRA1; MIM 603819) is a complex RNA molecule containing multiple stable stem-loop structures that functions in coactivation of nuclear receptors. SLIRP interacts with stem-loop structure-7 of SRA (STR7) and modulates nuclear receptor transactivation (Hatchell et al., 2006 [PubMed 16762838]).
Cite This Product
Anti-SLIRP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08297-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
81%
Moderate Homology
Rabbit
75%
Moderate Homology
Cow
74%
Moderate Homology
Sheep
73%
Moderate Homology
Mouse
72%
Moderate Homology
Dog
72%
Moderate Homology
Rat
71%
Moderate Homology
Zebrafish
45%
Low homology
Drosophila
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLIRP antibody
Immunohistochemistry (IHC) protocol for SLIRP antibody
Immunofluorescence (IF) protocol for SLIRP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SLIRP antibody
Flow Cytometry (FC) protocol for SLIRP antibody
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Product Promise
We promise Anti-SLIRP Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A08297-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026