Overview
| Product Name |
Anti-Smad3 (phospho-S425) Antibody
See more SMAD3 products |
| Catalog# |
A00059S425 |
| Pack Size |
100ul |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Smad3 (phospho-S425) Antibody catalog # A00059S425. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ul in Liquid form antibody. |
| Cite This Product |
Anti-Smad3 (phospho-S425) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00059S425)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
IHC
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
Gene/Protein Basic Information For SMAD3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P84022 |
|---|
| NCBI Gene Id | 4088 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | SMAD3 |
|---|
| Protein Name | Mothers against decapentaplegic homolog 3 |
|---|
| Superfamily | dwarfin/SMAD family |
|---|
| Alternative Names | SMAD3|DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HsT17436; JV15-2MGC60396; MAD homolog 3; mad protein homolog; MAD, mothers against decapentaplegic homolog 3 (Drosophila); MAD, mothers against decapentaplegic homolog 3; mad3; MADH3mad homolog JV15-2; mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3HSPC193; SMAD, mothers against DPP homolog 3 (Drosophila); SMAD, mothers against DPP homolog 3; Smad3 |
|---|
| Molecular Weight | 48081 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SMAD3, check out the SMAD3 Infographic
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In this infographic you will see the following information for SMAD3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the SMAD3 infographic
Our Boster Quality Guarantee for Anti-Smad3 (phospho-S425) Antibody covers its use in the following applications.
IHC: 1:50~1:200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Immunohistochemistry validation of SMAD3 using Anti-Smad3 (phospho-S425) Antibody (A00059S425).
Immunohistochemistry (IHC) analyzes of p-Smad3 (S425) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC
Figure 2. Western blotting validation for Anti-Smad3 (phospho-S425) Antibody A00059S425
Western blot (WB) analysis of p-Smad3 (S425) pAb at 1:500 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:Hela treated with EGF (0.1ng/ml
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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