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Product Info Summary
| SKU: | A03376 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SPTA1 Antibody
SKU/Catalog Number
A03376
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SPTA1 Antibody catalog # A03376. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SPTA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03376)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human alpha 1 Spectrin(NP_003117.2).
Reactive Species
A03376 is reactive to SPTA1 in Human, Mouse, Rat
Calculated molecular weight
280.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03376 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using alpha 1 Spectrin antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human tonsil using alpha 1 Spectrin antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded rat spleen using alpha 1 Spectrin antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Specific Publications For Anti-SPTA1 Antibody (A03376)
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