Product Info Summary
| SKU: | A04549 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Sulfhydryl oxidase 1 QSOX1 Antibody
SKU/Catalog Number
A04549
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Sulfhydryl oxidase 1 QSOX1 Antibody (Catalog # A04549). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
QSOX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Sulfhydryl oxidase 1 QSOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04549)
Host
Rabbit
Contents
QSOX1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
QSOX1 antibody was raised against a 17 amino acid peptide near the center of human QSOX1. The immunogen is located within amino acids 360 - 410 of QSOX1.
Cross-reactivity
QSOX1 antibody is human and mouse reactive. QSOX1 antibody is predicted to not cross-react with QSOX2.
Reactive Species
A04549 is reactive to QSOX1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
82.6 kDa
Background of QSOX1
The QSOX1 gene, also known as Quiescin Q6, is a fusion of two ancient genes: thioredoxin and ERV1. Its expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation (1). The QSOX1 protein oxidizes sulfhydryl groups to form disulfide bonds in proteins. QSOX1 expression is induced by hypoxia (2) and appears to protect cells against oxidative stress-induced apoptosis (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04549 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
QSOX1 antibody can be used for detection of QSOX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of QSOX1 in EL4 cell lysate with QSOX1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of QSOX1 in human spleen tissue with QSOX1 antibody at 5 μg/mL.
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Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.
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Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.
Specific Publications For Anti-Sulfhydryl oxidase 1 QSOX1 Antibody (A04549)
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