Click image to see more details
Product Info Summary
| SKU: | M04967 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334
SKU/Catalog Number
M04967
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334 (Catalog # M04967). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04967)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM334
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Surfactant protein A (SP-A).
Cross-reactivity
This antibody reacts to human Surfactant protein A (SP-A).
Reactive Species
M04967 is reactive to SFTPA2 in Human
Calculated molecular weight
26.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04967 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of human lung tissue lysate using anti-Surfactant protein A (SP-A) rabbit monoclonal antibody (Clone RM334) at a 1:10,000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using anti-Surfactant protein A (SP-A) rabbit monoclonal antibody (Clone RM334) at a 1:1000 dilution.
Specific Publications For Anti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334 (M04967)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Surfactant protein A (SP-A) Rabbit Monoclonal Antibody, Clone#RM334
Question
Will anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 M04967 work for WB with upper lobe of lung?
E. Taylor
Verified customer
Asked: 2020-02-27
Answer
According to the expression profile of upper lobe of lung, SFTPA2 is highly expressed in upper lobe of lung. So, it is likely that anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 M04967 will work for WB with upper lobe of lung.
Boster Scientific Support
Answered: 2020-02-27
Question
Is a blocking peptide available for product anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 (M04967)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do provide the blocking peptide for product anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 (M04967). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-13
Question
Is this M04967 anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 reactive to the isotypes of SFTPA2?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The immunogen of M04967 anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 is A peptide corresponding to the C-terminus of human Surfactant protein A (SP-A). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-06
Question
Does M04967 anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
It shows on the product datasheet, M04967 anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for upper lobe of lung using anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 M04967. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
Can you help my question with product M04967, anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Thomas
Verified customer
Asked: 2018-01-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04967 anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-05
Question
Is there a BSA free version of anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 M04967 available?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Surfactant protein A (SP-A) Rabbit Monoclonal antibody, Clone#RM334 M04967 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-28
