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Product Info Summary
| SKU: | A03667 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TBC1 domain family member 1 TBC1D1 Antibody
SKU/Catalog Number
A03667
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TBC1 domain family member 1 TBC1D1 Antibody (Catalog # A03667). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
TBC1D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TBC1 domain family member 1 TBC1D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03667)
Host
Rabbit
Contents
TBC1D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TBC1D1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human TBC1D1. The immunogen is located within the last 50 amino acids of TBC1D1.
Reactive Species
A03667 is reactive to TBC1D1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
133.1 kDa
Background of TBC1D1
TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03667 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TBC1D1 antibody can be used for detection of TBC1D1 by Western blot at 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TBC1D1 expression in (A) 293 and (B) Daudi cell lysate with TBC1D1 antibody at 2 μg/ml.
Specific Publications For Anti-TBC1 domain family member 1 TBC1D1 Antibody (A03667)
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