Anti-TCF3/E2A Antibody

Boster Bio Anti-TCF3/E2A Antibody catalog # A00095. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00095
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-TCF3/E2A Antibody

See all TCF-3/E2A products

SKU/Catalog Number

A00095

Size

100μl

Form

Liquid

Description

Boster Bio Anti-TCF3/E2A Antibody catalog # A00095. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TCF3/E2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00095)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from C-terminal of human TCF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00095 is reactive to TCF3 in Human, Mouse, Rat

Applications

A00095 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For TCF3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P15923

Gene ID

6929

Gene Name

TCF3

Full Name

Transcription factor E2-alpha

Weight

67.6kDa

Alternative Names

bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor TCF3 AGM8, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75 transcription factor 3 transcription factor E2-alpha|NOL1-TCF3 fusion|VDR interacting repressor|class B basic helix-loop-helix protein 21|helix-loop-helix protein HE47|immunoglobulin transcription factor 1|kappa-E2-binding factor|negative vitamin D response element-binding protein|transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)|transcription factor 3 variant 3|transcription factor ITF-1|vitamin D receptor-interacting repressor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TCF3, check out the TCF3 Infographic

TCF3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TCF3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-TCF3/E2A Antibody

Question

We are currently using anti-TCF3/E2A antibody A00095 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

The anti-TCF3/E2A antibody (A00095) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-18

Question

Is a blocking peptide available for product anti-TCF3/E2A antibody (A00095)?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

We do provide the blocking peptide for product anti-TCF3/E2A antibody (A00095). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-12

Question

Is there a BSA free version of anti-TCF3/E2A antibody A00095 available?

Verified Customer

Verified customer

Asked: 2018-08-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCF3/E2A antibody A00095 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-08-13

Question

Please see the WB image, lot number and protocol we used for testis using anti-TCF3/E2A antibody A00095. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-30

Question

Does anti-TCF3/E2A antibody A00095 work on horse WB with lymphoma?

A. Patel

Verified customer

Asked: 2015-11-27

Answer

Our lab technicians have not validated anti-TCF3/E2A antibody A00095 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TCF3/E2A antibody A00095 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse lymphoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-11-27

Question

My question regarding product A00095, anti-TCF3/E2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Carter

Verified customer

Asked: 2014-01-17

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00095 anti-TCF3/E2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-01-17

Question

Would anti-TCF3/E2A antibody A00095 work for IHC with testis?

L. Mangal

Verified customer

Asked: 2013-09-05

Answer

According to the expression profile of testis, TCF3 is highly expressed in testis. So, it is likely that anti-TCF3/E2A antibody A00095 will work for IHC with testis.

Boster Scientific Support

Answered: 2013-09-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A00095
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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