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Product Info Summary
| SKU: | A01032 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-FOXA2 Antibody
SKU/Catalog Number
A01032
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-FOXA2 Antibody (Catalog # A01032). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
FOXA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-FOXA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01032)
Host
Rabbit
Contents
FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 . The immunogen is located within amino acids 280 - 330 of FOXA2.
Cross-reactivity
At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.
Reactive Species
A01032 is reactive to FOXA2 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.3 kDa
Background of FOXA2
FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01032 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.
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Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.
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Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibody at 5 μg/mL.
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Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibody at 20 μg/mL.
Green: FOXA2 antibody (A01032)
Red: Phylloidin staining
Blue: DAPI staining
Specific Publications For Anti-FOXA2 Antibody (A01032)
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