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Product Info Summary
| SKU: | A30532 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TF3C2 Antibody
SKU/Catalog Number
A30532
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TF3C2 Antibody (Catalog# A30532). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TF3C2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30532)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TF3C2. Immunogen sequence location: 71-120
Reactive Species
A30532 is reactive to GTF3C2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
100.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30532 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using TFIIIC110 Polyclonal Antibody
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Western blot analysis of lysates from HepG2, COLO205, and HUVEC cells, using TF3C2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TF3C2 Antibody (A30532)
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1 Customer Q&As for Anti-TF3C2 Antibody
Question
We are currently using anti-TF3C2 antibody A30532 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The anti-TF3C2 antibody (A30532) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-14
