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Product Info Summary
| SKU: | PA1624 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TIM 1/HAVCR1 Antibody Picoband®
SKU/Catalog Number
PA1624
BA3536 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIM 1/HAVCR1 Antibody catalog # PA1624. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIM 1/HAVCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1624)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TIM 1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1624 is reactive to HAVCR1 in Human
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
39.3 kDa
Background of HAVCR1
KIM1 (KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1624 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: SMMC Cell, HELA Cell, PANC Cell, M231 Cell, M453 Cell,
IHC: Human Tonsil Tissue
ICC: K562 Cell
Validation Images & Assay Conditions
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Anti-TIM 1 antibody, PA1624, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: M453 Cell Lysate
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Anti-TIM 1 antibody, PA1624, IHC(P)
IHC(P): Human Tonsil Tissue
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Anti-TIM 1 antibody, PA1624, ICC
ICC: K562 Cell
Specific Publications For Anti-TIM 1/HAVCR1 Antibody Picoband® (PA1624)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TIM 1/HAVCR1 Antibody Picoband®
Question
We are currently using anti-TIM 1/HAVCR1 antibody PA1624 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
B. Jones
Verified customer
Asked: 2020-05-06
Answer
The anti-TIM 1/HAVCR1 antibody (PA1624) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
We have been able to see staining in human cervix carcinoma. Any tips? Is anti-TIM 1/HAVCR1 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
From literature cervix carcinoma does express HAVCR1. From Uniprot.org, HAVCR1 is expressed in adult mammalian kidney, liver, kidney, cervix carcinoma, among other tissues. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2019-06-27
Question
Our lab were satisfied with the WB result of your anti-TIM 1/HAVCR1 antibody. However we have seen positive staining in adult mammalian kidney membrane using this antibody. Is that expected? Could you tell me where is HAVCR1 supposed to be expressed?
M. Miller
Verified customer
Asked: 2017-05-17
Answer
According to literature, adult mammalian kidney does express HAVCR1. Generally HAVCR1 expresses in membrane. Regarding which tissues have HAVCR1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 9658108, 15372022
Boster Scientific Support
Answered: 2017-05-17
Question
Our lab used your anti-TIM 1/HAVCR1 antibody for ICC on kidney in the past. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining kidney as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
K. Baker
Verified customer
Asked: 2017-01-10
Answer
I have checked the website and datasheets of our anti-TIM 1/HAVCR1 antibody and it seems that PA1624 has been tested on human in both ICC and IHC. Thus PA1624 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-01-10
