SKU PA1076
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, IHC

Overview

Product Name Anti-TIMP2 Antibody
SKU/Catalog Number PA1076
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Metalloproteinase inhibitor 2(TIMP2) detection. Tested with IHC-P, ELISA in Human;Mouse;Rat.
Cite This Product Anti-TIMP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1076)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human TIMP2(205-220aa RGAAPPKQEFLDIEDP), identical to the related mouse and rat sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1076 1 IHC anti timp 2 antibody.jpg

Anti-TIMP2 antibody, PA1076, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P16035
Gene Name TIMP2
Protein Name Metalloproteinase inhibitor 2
Alternative Names Metalloproteinase inhibitor 2;CSC-21K;Tissue inhibitor of metalloproteinases 2;TIMP-2;TIMP2;
Subcellular Localization Secreted.
Molecular Weight 24399 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. .
Research Areas Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis / Ubiquitin, Signal Transduction

*You can search these to find other products in these research areas.
Background TIMP metallopeptidase inhibitor 2, a tissue inhibitor of metalloproteinases, also known as TIMP2, is a human gene, thought to be a metastasis suppressor. This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.

Other Recommended Resources

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Order Product (PA1076)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships in 7-10 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers
MMP-1/2 and TIMP-1/2 expression levels, and the levels of collagenous and elastic fibers correlate with disease progression in a hamster model of tongue cancer
Over-Expression of Calpastatin Inhibits Calpain Activation and Attenuates Post-Infarction Myocardial Remodeling
MiR-19a is correlated with prognosis and apoptosis of laryngeal squamous cell carcinoma by regulating TIMP-2 expression
Effect of norcantharidin on proliferation and invasion of human gallbladder carcinoma GBC-SD cells
Wu Ty, Zhang Th, Qu Lm, Feng Jp, Tian Ll, Zhang Bh, Li Dd, Sun Yn, Liu M. Int J Clin Exp Pathol. 2013 Dec 15;7(1):56-63. Ecollection 2014. Mir-19A Is Correlated With Prognosis And Apoptosis Of Laryngeal Squamous Cell Carcinoma By Regulating Timp-2...
Qiu Lb, Zhou Y, Wang Q, Yang Ll, Liu Hq, Xu Sl, Qi Yh, Ding Gr, Guo Gz. Toxicology. 2011 Jul 11;285(1-2):31-8. Doi: 10.1016/J.Tox.2011.03.019. Epub 2011 Apr 8. Synthetic Gelatinases Inhibitor Attenuates Electromagnetic Pulse-Induced Blood-Brain Ba...
Shan A, Zhou C, He Y, Feng W, Chen G, Zhong G. Ren Fail. 2013;35(1):37-42. Doi: 10.3109/0886022X.2012.741645. Epub 2012 Nov 14. Expression Of Both Matrix Metalloproteinase-2 And Its Tissue Inhibitor-2 In Tunica Media Of Radial Artery In Uremic Pat...
Hu P, Wang J, Zhao Xq, Hu B, Lu L, Qin Yh. Mol Biol Rep. 2013 Feb;40(2):1429-41. Doi: 10.1007/S11033-012-2186-7. Epub 2012 Oct 17. Overexpressed C-Type Natriuretic Peptide Serves As An Early Compensatory Response To Counteract Extracellular Matrix...
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon ...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to timp 2 antibody, timp2 antibody, timp-2 antibody