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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human COL1A1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1692)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1692 is reactive to COL1A1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK1692 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1692-CAP||Anti-Human COL1A1 Pre-coated 96-well strip microplate||1|
|EK1692-ST||Human COL1A1 Standard||2 vials, 10 ng/tube|
|EK1692-DA||Human COL1A1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human COL1A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect COL1A1, Dilution ratio of 1:2000, concentration in serum is 594-1000ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Pro-collagen I alpha 1/COL1A1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of COL1A1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For COL1A1 (Source: Uniprot.Org, NCBI)
Collagen alpha-1(I) chain
fibrillar collagen family
Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COL1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1692 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
EK1692 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA
MiR-200a ameliorates peritoneal fibrosis and functional deterioration in a rat model of peritoneal dialysis Xin Wei, et al. Int Urol Nephrol. 2019; 51(5): 889–896. Published online 2019 Mar 19. doi: 10.1007/s11255-019-02122-4
Murine and Chinese cobra venom%u2011derived nerve growth factor stimulate chondrogenic differentiation of BMSCs in vitro: A comparative study
Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.
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