Human TIMP-2 PicoKine® Quick ELISA Kit

TIMP-2 ELISA kit, 120-minute protocol

Human TIMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0522
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human TIMP-2 PicoKine® Quick ELISA Kit

See all TIMP-2 products

SKU/Catalog Number

FEK0522

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TIMP2 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TIMP-2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0522)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: C27-P220

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0522 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0522 is reactive to TIMP2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

FEK0522 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP-2

TIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Kit Components

Catalog number Description Quantity
FEK0522-CAP 96-well plate precoated with anti-Human TIMP-2 antibody 1
EK0522-ST lyophilized recombinant Human TIMP-2 standard 10 ng/tube
FEK0522-DA Biotinylated anti-Human TIMP-2 detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0050.1090.1980.4180.7871.3972.1092.513

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2491786439923916544356
Standard deviation17.4%394.65325.5216.73112.47361.54
CV (%)6.4%5.3%7.4%7%6.8%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TIMP-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124925827529126816.116%
Sample 21786179817962014184895.655.1%
Sample 343995066450650164746297.196.2%
*number of samples for each test n=16.

Gene/Protein Information For TIMP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P16035

Gene ID

7077

Gene Name

TIMP2

Full Name

Metalloproteinase inhibitor 2

Weight

24.399kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2 TIMP2 CSC-21K, DDC8 TIMP metallopeptidase inhibitor 2 metalloproteinase inhibitor 2|testicular secretory protein Li 57|tissue inhibitor of metalloproteinases 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP2, check out the TIMP2 Infographic

TIMP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

FEK0522 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...

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2 Customer Q&As for Human TIMP-2 PicoKine® Quick ELISA Kit

Question

Do you have information regarding the target epitope for the antibody in your TIMP2 ELISA kit?

Verified customer

Asked: 2019-04-22

Answer

Unfortunately, we do not do epitope mapping for our antibodies, so we are only able to provide the immunogen sequence.

Boster Scientific Support

Answered: 2019-04-22

Question

Could you give me more information about FEK0522? Its synthetic peptide derived from human TIMP2 N-term or C-term? Or what is a rough amino acid range?

Verified Customer

Verified customer

Asked: 2017-05-04

Answer

The FEK0522 specific immunogen is consider as proprietary. However, FEK0522 antibody binds to the N-terminal domain of the protein.

Boster Scientific Support

Answered: 2017-05-04

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