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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-TLR2 Antibody Picoband™
Boster Bio Anti-TLR2 Antibody Picoband™ catalog # A00131. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00131)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human TLR2 recombinant protein (Position: H610-S784). Human TLR2 shares 87.4% amino acid (aa) sequence identity with mouse TLR2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00131 is reactive to TLR2 in Human, Mouse, Rat
A00131 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (A00131).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (Catalog # A00131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 89KD. The expected band size for TLR2 is at 89KD.
Gene/Protein Information For TLR2 (Source: Uniprot.Org, NCBI)
Toll-like receptor 2
Toll-like receptor family
CD282 antigen; CD282; TIL4CD282; TLR2; toll/interleukin 1 receptor-like 4; Toll/interleukin-1 receptor-like protein 4; toll-like receptor 2 TLR2 CD282, TIL4 toll like receptor 2 toll-like receptor 2|toll/interleukin-1 receptor-like protein 4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TLR2, check out the TLR2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TLR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00131 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Feng S,Ju L,Shao Z,Grzanna M,Jia L,Liu M.Therapeutic Effect of C-C Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis.J Inflamm Res.2020 Jul 21;13:343-356.doi:10.2147/JIR.S254717.PMID: 32801828;PMCID:PMC7398876.
A00131 usage in article: APP:WB, SAMPLE:SPLEEN, DILUTION:NA
Expression of TLR2/4 on Epididymal Spermatozoa of the Chinese Soft%u2010Shelled Turtle Pelodiscus sinensis During the Hibernation Season
Inhibition of Zymosan-Induced Inflammatory Factors Expression by ATRA Nanostructured Lipid Carriers
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and?Staphylococcus aureus
Wang Y, Ge P, Yang L, Wu C, Zha H, Luo T, Zhu Y. J Neuroinflammation. 2014 Jan 24;11:15. Doi: 10.1186/1742-2094-11-15. Protection Of Ischemic Post Conditioning Against Transient Focal Ischemia-Induced Brain Damage Is Associated With Inhibition Of ...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
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