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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-RANKL/TNFSF11 Antibody Picoband™
Boster Bio Anti-RANKL/TNFSF11 Antibody Picoband™ catalog # PB10015. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANKL/TNFSF11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10015)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived rat RANKL recombinant protein (Position: F141-D318). Rat RANKL shares 87.9% and 96.6% amino acid (aa) sequence identity with human and mouse RANKL, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB10015 is reactive to Tnfsf11 in Mouse, Rat
PB10015 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RANKL using anti-RANKL antibody (PB10015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANKL antigen affinity purified polyclonal antibody (Catalog # PB10015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANKL at approximately 40 kDa. The expected band size for RANKL is at 35 kDa.
Gene/Protein Information For Tnfsf11 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 11
tumor necrosis factor family
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 Tnfsf11|RANKL|TNF superfamily member 11|tumor necrosis factor ligand superfamily member 11|ODF|OPGL|TNF-related activation-induced cytokine|TRANCE|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa-B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor superfamily member 11*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfsf11, check out the Tnfsf11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB10015 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hu Y,Hao X,Liu C,Ren C,Wang S,Yan G,Meng Y,Mishina Y,Shi C,Sun H.Acvr1 deletion in osteoblasts impaired mandibular bone mass through compromised osteoblast differentiation and enhanced sRANKL-induced osteoclastogenesis. J Cell Physiol.2020 Nov 29.doi:10.1
Effectiveness of combined salmon calcitonin and aspirin therapy for osteoporosis in ovariectomized rats
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