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Product Info Summary
| SKU: | A00340-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-NFAT2/NFATC1 Antibody Picoband®
SKU/Catalog Number
A00340-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFAT2/NFATC1 Antibody Picoband® catalog # A00340-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFAT2/NFATC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00340-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00340-1 is reactive to NFATC1 in Human
Observed Molecular Weight
101 kDa
Calculated molecular weight
101.2 kDa
Background of NFATC1
NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00340-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Jurkat whole cell, human Daudi whole cell, human Ramos whole cell, human Raji whole cell
FCM: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of NFAT2/NFATC1 using anti-NFAT2/NFATC1 antibody (A00340-1).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Ramos whole cell lysates,
Lane 4: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2/NFATC1 antigen affinity purified polyclonal antibody (A00340-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFAT2/NFATC1 at approximately 101 kDa. The expected band size for NFAT2/NFATC1 is at 101 kDa.
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Flow Cytometry analysis of Jurkat cells using anti-NFAT2/NFATC1 antibody (A00340-1).
Overlay histogram showing Jurkat cells stained with A00340-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFAT2/NFATC1 Antibody (A00340-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-NFAT2/NFATC1 Antibody Picoband® (A00340-1)
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Customer Q&As
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4 Customer Q&As for Anti-NFAT2/NFATC1 Antibody Picoband®
Question
Our lab used your anti-NFAT2/NFATC1 antibody for Flow Cytometry on b-cell last year. I am using rat, and We intend to use the antibody for WB next. I am interested in examining b-cell as well as peripheral blood lymphocyte t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
I have checked the website and datasheets of our anti-NFAT2/NFATC1 antibody and I see that A00340-1 has been tested on rat in both Flow Cytometry and WB. Thus A00340-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-17
Question
My boss were happy with the WB result of your anti-NFAT2/NFATC1 antibody. However we have observed positive staining in b-cell lymphoma cytoplasm. using this antibody. Is that expected? Could you tell me where is NFATC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
Based on literature, b-cell lymphoma does express NFATC1. Generally NFATC1 expresses in cytoplasm. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2018-05-24
Question
We are currently using anti-NFAT2/NFATC1 antibody A00340-1 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-29
Answer
The anti-NFAT2/NFATC1 antibody (A00340-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-29
Question
We have been able to see staining in mouse b-cell lymphoma. Any tips? Is anti-NFAT2/NFATC1 antibody supposed to stain b-cell lymphoma positively?
E. Li
Verified customer
Asked: 2017-01-06
Answer
Based on literature b-cell lymphoma does express NFATC1. Based on Uniprot.org, NFATC1 is expressed in muscle organ, peripheral blood lymphocyte t-cell, b-cell, b-cell lymphoma, brain, erythroleukemia, among other tissues. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2017-01-06
