Product Info Summary
SKU: | EZ0411 |
---|---|
Size: | For 5 plates, 96 wells each, reagents only, plates are not included |
Reactive Species: | Mouse |
Application: | ELISA |
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Product info
Product Name
Mouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
SKU/Catalog Number
EZ0411
Size
For 5 plates, 96 wells each, reagents only, plates are not included
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0411)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: F25-T211
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0411 is reactive to IL6 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EZ0411 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0411-CA | Rat anti- mouse il6 monoclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0411-DA | Biotinylated goat anti- mouse il6 polyclonal antibody (Detection Antibody) | 500 µl, 4.7 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0411-ST | Lyophilized recombinant mouse il6 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.032 | 0.085 | 0.141 | 0.237 | 0.452 | 0.816 | 1.470 | 2.281 |
Data Example Images
Click image to see more details
Mouse IL-6 EZ Set ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For IL6 (Source: Uniprot.Org, NCBI)
Gene Name
IL6
Full Name
Interleukin-6
Weight
23.718kDa
Superfamily
IL-6 superfamily
Alternative Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF2; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ0411)
Hello CJ!
EZ0411 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
%u516D%u541B%u5B50%u6C64%u63D0%u53D6%u7269%u6291%u5236%u98DF%u7BA1%u764C Ec9706 %u7EC6%u80DE%u589E%u6B96%u53CA%u5176%u5BF9%u4FE1%u53F7%u8F6C%u5BFC%u548C%u8F6C%u5F55%u6D3B%u5316%u56E0%u5B50 3 %u4FE1%u53F7%u901A%u8DEF%u7684%u5F71%u54CD
Novel therapeutic approach by nicotine in experimental model of multiple sclerosis
Sialic acid-modified solid lipid nanoparticles as vascular endothelium-targeting carriers for ischemia-reperfusion-induced acute renal injury
Levels of serum pentraxin 3, IL-6, fetuin A and insulin in patients with rheumatoid arthritis
Changes in mediators of inflammation and pro-thrombosis after 12 months of dietary modification in adults with metabolic syndrome
From the Cover: Lung-Specific Overexpression of Constitutively Active IKK2 Induces Pulmonary and Systemic Inflammations but Not Hypothalamic Inflammation and %u2026
E-selectin-targeted sialic acid-PEG-dexamethasone micelles for enhanced anti-inflammatory efficacy for acute kidney injury
Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial
Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice
TNF?-Mediated Loss of ?-Catenin/E-Cadherin Association and Subsequent Increase in Cell Migration Is Partially Restored by NKX3.1 Expression in Prostate Cells
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Mouse IL-6 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
Question
For the Mouse IL-6 ELISA Kit EZ-Set, what types of tissues have been tested? Have you tested mouse brain? Is so, you have any references?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
The Mouse IL-6 ELISA Kit EZ-Set have been tested successfully in liver lysate but not yet in brain tissue. Thus we cannot give you further information about it.
Boster Scientific Support
Answered: 2017-12-20