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Product Info Summary
| SKU: | M00434-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: OTI3B9]
View all Topoisomerase I Antibodies
SKU/Catalog Number
M00434-1
Size
100 µl
Description
Boster Bio TOP1 mouse monoclonal antibody,clone OTI3B9. Catalog# M00434-1. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: OTI3B9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00434-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3B9
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 93-151 of human TOP1 (NP_003277 ) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00434-1 is reactive to TOP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
90.726kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00434-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP1 (1:2000).
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Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-TOP1 monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For TOP1 (Source: Uniprot.org, NCBI)
Gene Name
TOP1
Full Name
DNA topoisomerase 1
Weight
90.726kDa
Superfamily
type IB topoisomerase family
Alternative Names
DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 |topoisomerase (DNA) I|type I DNA topoisomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP1, check out the TOP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: OTI3B9] (M00434-1)
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