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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse TNFSF4/OX40L ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse TNFSF4/OX40L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFSF4/OX40L ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0858)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S51-L198
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0858 is reactive to Tnfsf4 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0858 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of OX40 Ligand/TNFSF4
OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al. (1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
|EK0858-CAP||Anti-Mouse Tnfsf4 Pre-coated 96-well strip microplate||1|
|EK0858-ST||Mouse Tnfsf4 Standard||2 vials, 10 ng/tube|
|EK0858-DA||Mouse Tnfsf4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse TNFSF4/OX40L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect OX40 Ligand/TNFSF4, Dilution ratio of 1:1, concentration in serum is NO-30pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFSF4/OX40L ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of OX40 Ligand/TNFSF4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Tnfsf4 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 4
tumor necrosis factor family
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 Tnfsf4|Ath, Ath-, Ath-1, Ath1, CD134, CD134L, OX-40L, OX4, Ox40l, TXGP1, Tnlg2b, Txgp, Txgp1l, gp3, gp34|tumor necrosis factor (ligand) superfamily, member 4|tumor necrosis factor ligand superfamily member 4|OX40 ligand|atherosclerosis 1|tax-transcriptionally activated glycoprotein 1 ligand|tumor necrosis factor ligand 2b*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfsf4, check out the Tnfsf4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0858 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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