Mouse TNFSF4/OX40L ELISA Kit PicoKine™

Mouse TNFSF4/OX40L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0858
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TNFSF4/OX40L ELISA Kit PicoKine™

See all OX40 Ligand/TNFSF4 products

SKU/Catalog Number

EK0858

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFSF4/OX40L ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TNFSF4/OX40L ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0858)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S51-L198

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0858 is reactive to Tnfsf4 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0858 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0858-CAP Anti-Mouse Tnfsf4 Pre-coated 96-well strip microplate 1
EK0858-ST Mouse Tnfsf4 Standard 2 vials, 10 ng/tube
EK0858-DA Mouse Tnfsf4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0340.2530.3670.5521.0101.5222.0602.486

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect OX40 Ligand/TNFSF4, Dilution ratio of 1:1, concentration in serum is NO-30pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFSF4/OX40L ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)17656220821605122260
Standard deviation10.3836.81141.5710.8838.91187.58
CV (%)6.8%6.8%6.8%6.8%7.6%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of OX40 Ligand/TNFSF4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 117617415114916212.537.7%
Sample 256259651058856433.615.9%
Sample 3208217681972207119731266.3%
*number of samples for each test n=16.

Gene/Protein Information For Tnfsf4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P43488

Gene ID

22164

Gene Name

Tnfsf4

Full Name

Tumor necrosis factor ligand superfamily member 4

Weight

22.255kDa

Superfamily

tumor necrosis factor family

Alternative Names

CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 Tnfsf4|Ath, Ath-, Ath-1, Ath1, CD134, CD134L, OX-40L, OX4, Ox40l, TXGP1, Tnlg2b, Txgp, Txgp1l, gp3, gp34|tumor necrosis factor (ligand) superfamily, member 4|tumor necrosis factor ligand superfamily member 4|OX40 ligand|atherosclerosis 1|tax-transcriptionally activated glycoprotein 1 ligand|tumor necrosis factor ligand 2b

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfsf4, check out the Tnfsf4 Infographic

Tnfsf4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfsf4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse TNFSF4/OX40L ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated OX40 Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the OX40 Ligand antigen?

H. Lee

Verified customer

Asked: 2020-06-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-06-22

Question

Q: how do I analyze ELISA data? I have obtained OX40 Ligand level in serum.

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-04

Question

I purchased your EK0858 Mouse TNFSF4/OX40L ELISA Kit for IHC/IHC-Fr. Could you possibly share with me some images? Cheers!

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

The product EK0858 Mouse TNFSF4/OX40L ELISA Kit was being tested using frozen human inflammatory lymph nodes by immunofluorescence resulting positive subgroup cells. We do not have an image of this EK0858 antibody in IHC-Fr.

Boster Scientific Support

Answered: 2020-03-03

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of OX40 Ligand in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

L. Varma

Verified customer

Asked: 2019-09-12

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the OX40 Ligand ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-09-12

Question

Q: is it okay to use citrate plasma as samples in Mouse OX40 Ligand Picokine® ELISA Kit (Catalog # EK0858)?

W. Williams

Verified customer

Asked: 2019-07-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of OX40 Ligand causing degradation of its protein structure. OX40 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the OX40 Ligand ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-07-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-06-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-06-22

Question

Q: which procedure should I follow in order to thaw whole blood sample for OX40 Ligand ELISA after freezing?

S. Roberts

Verified customer

Asked: 2019-06-10

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test OX40 Ligand for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-06-10

Question

Q: Can OX40 Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-11-12

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