Anti-Transcription factor E2F1 E2F1 Antibody

Product Name

Anti-Transcription factor E2F1 E2F1 Antibody

View all E2F-1 Antibodies

SKU/Catalog Number

A00257-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Transcription factor E2F1 E2F1 Antibody catalog # A00257-1. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transcription factor E2F1 E2F1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00257-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00257-1 is reactive to E2F1 in Human, Mouse, Rat

Applications

A00257-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.92kDa

Background of E2F-1

HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of E2F1 using Anti-Transcription factor E2F1 E2F1 Antibody (A00257-1).

ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Transcription factor E2F1 E2F1 Antibody A00257-1

Western blot analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1

Gene/Protein Information For E2F1 (Source: Uniprot.org, NCBI)

Alternative Names

E2F transcription factor 1; E2F1; E2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; transcription factor E2F1 E2F1 E2F-1, RBAP1, RBBP3, RBP3 E2F transcription factor 1 transcription factor E2F1|PBR3|PRB-binding protein E2F-1|RBAP-1|RBBP-3|retinoblastoma-associated protein 1|retinoblastoma-binding protein 3

For more info on E2F1, check out the E2F1 Infographic

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In this infographic, you will see the following information for E2F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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