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- Table of Contents
E2F1 antibody (M00257) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-E2F1 Rabbit Monoclonal Antibody
E2F1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
E2F1 antibody was positively detected by WB in:
human HeLa whole cell lysates, human HepG2 whole cell, human U2OS whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
E2F1 antibody was positively detected by IHC in:
human spleen tissue, human colon adenocarcinoma tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, human placenta tissue, human testis cancer tissue, human thyroid papillary carcinoma tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-E2F1 Rabbit Monoclonal Antibody
View all E2F1 Antibodies
View all E2F1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IGD-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human E2F1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
65-70 kDa
Calculated Molecular Weight
46.9 kDa
Cite This Product
Anti-E2F1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00257)
Protocols
Western Blot protocol for E2F1 antibody
Immunohistochemistry (IHC) protocol for E2F1 antibody
Publications
Application:
Species:
Arthrocolin B Impairs Adipogenesis via Delaying Cell Cycle Progression During the Mitotic Clonal Expansion Period
Published: 2025-02-10
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Mouse
PMID: 40003939
U2 small nuclear RNA auxiliary factor 2, transcriptionally activated by the transcription factor Dp-1/E2F transcription factor 1 complex, enhances the growth and aerobic glycolysis of leiomyosarcoma cells
Published: 2022-05-03
Journal: Bioengineered
Application: WB,IF,CoIP
Impact Factor: 6.83
Species: Human
PMID: 35502531
Regulating A549 cells growth by ASO inhibiting miRNA expression
Published: 2010-01-05
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: IF
Impact Factor: 1.90
Species: Human
PMID: 20049626
Interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma
Published: 2009-12-29
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: IHC
Impact Factor: 0.00
Species: Human
PMID: 20037816
Review
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Product Promise
We promise Anti-E2F1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00257
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026