Transcription factor E2F1 Antibodies

Transcription factor E2F1 (E2F1)

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase.

E2F1 binds preferentially RB1 in a cell-cycle dependent way. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

Gene Information

Human
Gene Name:	E2F1
Uniprot:	Q01094
Entrez:	1869

Family

Belongs to:
E2F/DP family

Alternative Names

E2F transcription factor 1; E2F1; E2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; transcription factor E2F1

Molecular Weight

Mass (kDA):
46.92 kDA

Genomic Context

Human
Location:	20q11.22
Sequence:	20; NC_000020.11 (33675477..33686385, complement)

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Retinoblastoma
• Carcinoma
• Cell Transformation, Neoplastic
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Hyperplasia
• Malignant Paraganglionic Neoplasm
• Growth Arrest
• Carcinogenesis
• Malignant Neoplasm Of Prostate

• Prostatic Neoplasms
• Leukemia
• Liver Carcinoma
• Osteosarcoma
• Lung Neoplasms
• Adenocarcinoma
• Malignant Neoplasm Of Lung

Interacting Proteins

• BTG3
• CDK7
• CDK8
• DDB2
• HDAC1

• KAT2A
• MCPH1
• NCOR2
• PARP1
• PAWR

• PRMT5
• RB1
• RRP1B
• SIRT1
• Sirt1

• SP1
• STAT1
• TFDP1
• TOPBP1
• TRIM16

Pathways

• Cell Cycle
• Cell Proliferation
• S Phase
• Cell Growth
• Cell Death
• Cell Cycle Arrest
• Dna Replication
• Induction Of Apoptosis
• Localization
• Dna Repair
• G1 Phase
• Methylation
• Senescence

• Cell Division
• Cell Differentiation
• Mitosis
• Pathogenesis
• Rna Interference
• Oncogenesis
• Angiogenesis

PTMs

• Phosphorylation
• Methylation
• Acetylation
• Dephosphorylation
• Cleavage
• Ubiquitination
• Demethylation

• Deacetylation
• Reduction
• Oxidation
• Sumoylation
• Phosphorothioation
• Amidation
• Glycosylation

Research Areas

• Apoptosis
• Cancer
• Cell Cycle and Replication
• Phospho-Specific
• Transcription Factors and Regulators

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/E2F1

Best Uses of the E2F1 Marker

The E2F1 marker is utilized in multiple areas of biology and medical research. It is a molecule that plays an essential function in the production of the hormone insulin, the E2F1 molecule is crucial in understanding the human body. The protein is found in the bloodstream and has an impact on the body's glucose levels as well as the levels of lipids. If it's not properly expressed, it can cause numerous health problems.

Molecular Target

The E2F1 Molecular Target Marker is a key transcription element that is present in the nucleus of many cell kinds. It binds to DNA through dimerization with two other proteins and is located at the promoter region of various genes. It is a downstream target for Rb pocket protein, master regulator of cell cycle and major tumor suppressor. Rb preferentially connects to E2F subunits.

The loss of the E2F1 and E2F3 markers prevents MEFs from entering S-phase following the overexpression of the cMyc protein. The loss of either both markers affects S-phase entry and differentiation of MEFs. These proteins are crucial for the death of cells induced by activation. So, E2F1 and p73 play vital roles in the control of the progression of the cell cycle.

Researchers have discovered that E2F1 could play a key function in many forms of cancer. It regulates cell proliferation and apoptosis among other things. It also plays an important role in apoptopopulation. It is therefore essential to identify the molecular target of The E2F1 marker. It can aid doctors in determining the most effective treatment for the disease once it is discovered.

E2F1 is a marker for cancer. It also aids in cell differentiation, also known as EMT in SCLC cells. It is not known how E2F1 enhances EMT within SCLC cells. The gene also targets ZEB2 as an CDh2 inhibitory transcription factor. Although the Molecular Target Of E2F1 marker is effective but it isn't completely known how it works to promote this process.

Cellular Function

The Cellular Function of E2F1 gene is associated insulin resistance. It is induced via the circulating free fat acids. It is also associated with cardiovascular risk, since it inhibits cardiac neovascularization via regulating the expression of VEGF and PIGF. However, the function of cells of E2F1-/ mice is enhanced following myocardial infarction in a mouse model, indicating that increased E2F1 activity is a contributory factor in cardiomyopathy.

RNA-seq was used to analyze osteosarcoma cells with stable knockdowns of E2F1 or DDR1. The heat map showed that DDR1 abundance was significantly reduced after the E2F1-silence. We noticed a dramatic drop in DDR1 levels , both at the protein and mRNA levels in osteosarcoma cell lines. We cloned the DDR1 promoter region into Luciferase, to further study the role of E2F1 for osteosarcoma cell proliferation.

The proliferation of cells caused by hypoxia has been associated with a decrease in the expression of the E2F1 gene. Hypoxia-induced E2F1 expression may contribute to hypoxic pulmonary remodeling. This effect could be beneficial in the treatment of hypoxia-induced lung vascular remodeling in chickens, by inhibiting E2F1. To determine the significance of E2F1 and hypoxia-induced vascular remodeling, further research is needed.

The E2F1 gene is involved in a variety of aspects of the cell cycle's development. It regulates the metabolic functions of differentiated organs and is a contributor to global metabolism homeostasis. Its activity is increased in obesity, which can cause a number of problems. The pathways involved in obesity are highlighted in green, and those repressed in red. It is important to remember that E2F1 expression affects both cancer and the development of diabetes.

Clinical Applications

The E2F1 marker is highly sensitive and specific for apoptosis is highly sensitive. The E2F1 protein level is triggered when neurons or glial cells are cut off, resulting in an increase in the rate of apoptosis. Clinical applications of this marker have been described including Parkinson's disease, and neurodegenerative diseases. This protein is found in many tissues, including the nervous systems.

The E2F1 gene plays an essential function in the mTOR signaling pathway. It is involved in many types and types of cancer. E2F1 enhances the activity of mTORC1 as well as the expression of lysosomal-v-ATPase which is a key regulator of autophagy. While the exact function of E2F1 in autophagy remains a mystery, numerous studies highlight the crosstalk between E2F1 activity and other signaling pathways.

The increased expression of E2F1 has been reported in various model objects including crayfish VNCs , as well as rats with axotomized tails. The protein is found in the cytoplasm as well in the mechanoreceptor's nuclei. neurons in vertebrates. Rabbit antibodies to E2F1 protein recognized the same epitopes of the crayfish protein.

E2F1 expression can predict breast cancer patient outcome. It has been shown to be comparable with other predictors of gene expression. It also indicates that cancer cells have a favorable prognosis regardless of their status with respect to estrogen receptors or ERBB2. This study highlights the potential of targeted cancer treatments by controlling the metabolism of tumors. This new marker is an excellent supplement to any drug regimen.

Research has revealed that E2F1 is a major role in the development and maintenance of many metabolic disorders, such as cancer. The E2F1 gene is highly expressed in a variety of metabolic tissues during obesity and could contribute to the development of some of these pathological conditions. Thus, the E2F1 marker could have clinical applications in cancer, diabetes and obesity. Further research will help determine how E2F1 expression may predict the prognosis and the progression of disease.

Biologically Relevant

The E2F1 protein is expressed abundantly in NSCLC. However however, there is no definitive link between E2F1 expression and overall survival or freedom from progression. The protein's expression is associated with numerous applications in cancer research, including monitoring the recurrence of tumors. Read on to find out more about the most recent developments. The potential applications are described in this article.

One study highlights the functional significance of E2F1 in male fertility. Their study found that the E2F1 gene was associated with testicular development with point mutations and CNVs. The gene also regulates the process of apoptosis, which is a key process in germ cell development. This study also highlights the advantages of using E2F1 in cancer research.

E2F1 has been found to regulate several other genes involved in research into cancer. It also regulates the activity of antioxidant enzymes like MnSOD and FOXO3, which protect cells from damage by free radicals. Additionally, E2F1 has been linked to tumorigenesis. E2F1 may also play a role during cell senescence.

The E2F1 gene belongs to the E2F family of transcription factors. It regulates cell growth and gene expression and is associated with the RB protein. These two proteins are involved in the p53 dependent and -dependent cell death pathways, respectively. It is therefore possible that the SENP3-E2F1 axis could be beneficial for breast cancer research.

The tumor-suppressed FOXO3 gene blocks the expression of miR-377. miR-342-3p and miR-377. The E2F1 gene is a significant target for cancer research because it is important for regulating cell proliferation. It is essential for the survival of cells and regulates a variety of growth factors and hormones in cancer cells.

Cost

The E2F1 marker has shown promise in several studies. Its high rate of nuclear positivity is linked with aggressive behavior in malignant gastrointestinal tumors. The cost of this marker is unknown, but it's worth looking into. This research is based on NKI data. However, it's not available to everyone. In the meantime it's still too early to say whether the test is useful in every situation.

The clinical trial of the E2F1 marker has a significant impact on the cost. While many cancers are difficult to treat, it is a key prognostic factor. One recent study found that the gene can predict the extent of the cancer in rectal cancer. Researchers believe that the price of E2F1 will continue to go down as the effectiveness of cancer is determined. This is especially true for patients are at a high risk of dying due to the disease.

Researchers also discovered that E2F1 levels were tightly linked to prognosis. The authors discovered a number of similarities between the signature of 70 genes and the expression of a tumor marker. Particularly those with low E2F1 expression levels were classified as having a favorable prognosis. Similarly, patients with low E2F1 levels were also ER-positive. The researchers also examined E2F1 expression levels with expression profiles from the microarray data set from The Netherlands Cancer Institute.